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A 2500 base-pair long cDNA of unknown sequence was cloned into the MCS of a plasmid vector that has universal priming sequences adjacent to and on both sides of the MCS. On one side this is called the "forward universal priming sequence" and on the other it is called the "reverse universal priming sequence." Sanger sequencing was done using primers that are specific for each of these universal priming sequences and approximately 700 nucleotides of sequence was determined from each primer. Assuming it is not possible to obtain sequence analyses longer than 700 nucleotides, describe the steps that must be done to obtain the remainder of the sequence of the cloned cDNA.
Hint: There are two very different methods that will accomplish this. One method requires much more effort and is less reliable than the other. Either method will receive full credit.
For in case, it is well established that in presynaptic neurons, the elevation of cytosolic calcium triggers the fusion of exocytotic vesicles that contain neurotransmitters to the plasma membrane.
Yet jam can be left unrefrigerated for long periods without contamination by bacteria and mold. Which provides the best explanation for this phenomenon.
Which of the subsequent statements is true about phylogeny. The ancestral group frequently has all the synapomorphies of the descendant species.W hat phenotypes would be seen in the f2 and in what proportion.
What techniques would you utilize to detect this DNA sequence. Mechanical stress or trauma frequently triggers the symptoms of gouty arthritis in susceptible people. In this view elucidate why gouty arthritis affects the great toe more commonly than ..
Approximately half of our vitamin K is synthesized by bacteria in the colon. Based on this information, why might taking a broad spectrum antibiotic produce frequent nosebleeds.
Describe how vestigial structures provide clues about a species' evolutionary past. Illustrate the argument with vestigial structures found in humans or other real species.
Which of the following statements is true? The first cervical vertebra is called the axis.The spinal cord protects the vertebral column.
Substrate-level phosphorylation occurs within the metabolic pathway where sufficient energy is released by given chemical reaction to drive the synthesis of ATP from ADP and phosphate. In which the metabolic pathways are substrate-level phosphorylati..
Complete a chart by identifying six actions that reduce the risk of infection and strengthen the body's immune system. In the second column, explain how the action reduces risk of infection and strengthens the body's immune system.
Some wines are marketed as "sparkling" wines. Sparkling wines are considered so because of a dissolved gas that makes them fizzy. Concern what you know about the procedure of respiration to explain the type and origin of this gas.
The resulting f1 generation consists of all tall pink flowered plants. Assume that height and flower colour is each determined by a single gene locus on different chromosomes; predict the results of an f1 cross of dihybrid plants. Select appropriate ..
You are designing a new single-celled organism. Discuss the problems of size, getting molecules like nutrients and wastes in and out, temperature, energy and replication of genetic materials.
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