Explain hydrophobic interaction chromatography

Assignment Help Biology
Reference no: EM1398525

What is Hydrophobic interaction chromatography? and how do high salt, medium salts, and no salt buffers paly a role in the purification of GFP? I know that no salt brings polar amino acids to the surface of GFP and nopolar amino acids move inside but not sure about high or medium salt

Reference no: EM1398525

Questions Cloud

Could he be the father : A woman with blood type B has a baby with blood type O. A man she says is the baby's father has blood type A, but he denies having fathered the child. Could he be the father?
Which mode of action of an antiobiotic is least likely : which mode of action of an antiobiotic is least likely to cause side of effects in a ptient.
Explain kohlberg-s stages of moral development : Analyze your own life and try to set-up as honestly and accurately as possible where values have come from. Where might you fit in Kohlberg's Stages of Moral Development?
Find normal protein coded for by the mrna in the mixture : In an experiment, you purify a cysteine-charged tRNA and chemically alter the amino acid attached to it converting it to alanine.
Explain hydrophobic interaction chromatography : What is Hydrophobic interaction chromatography? and how do high salt, medium salts, and no salt buffers paly a role in the purification of GFP? I know that no salt brings polar amino acids to the surface of GFP
Comparing the results of two calculations of concentration : Molecular Weights as determined by SDS-PAGE & NATIVE PAGE GEL How do the two masses compare with one another as determined by the two methods?
Estimate the pressure gradient required to maintain cardiac : A patient has a stenotic mitral valve which has a reduced effective orifice area of 1.0 cm^2. The systolic ejection period is 330 milliseconds with a heart rate of 70 beats/min.
Develop a routine assay for the virus : What aspects of modern society increase the opportunities for a global pandemic with H7N9 and Describe the key molecular/cellular events in the HIV lifecycle from entry to assembly and exit from the host cell.
Which reaction releases more free energy : Which person would tend to have a higher proportion of slow-twitch than fast-twitch muscles: a weight lifter, a sprinter, or a long distance runner?

Reviews

Write a Review

Biology Questions & Answers

  Maximum theoretical number of water molecules

Determine the maximum theoretical number of water molecules that one urea molecule can hydrogen bond with?

  How crossing affect the outcome of meiosis

Suppose that an organism exists in which crossing over does not occur, but that all other processes associated with meiosis occur normally.

  What is the step-by-step explanation

The carbonic anhydrase in proximal tubule epithelial cells are particularly sensitive to inhibition by the medication, "Topamax." What is the step-by-step explanation of how the inhibitory action of how Topamax caused the disturbances in a person'..

  What percentage of their offspring will carry the gene

For silkworms, C represents the dominant allele for a yellow cocoon and c represents the recessive allele for a white cocoon. Draw a punnett square crossing a homozygous dominantparent with a homozygous recessive parent. What percentage of the off..

  Functional unit of nerve tissue

Discuss and define the functional unit of nerve tissue and what are the three types of muscle cells and what is their general function/characteristic?

  Predictions for the ecori digest of lambda dna

Assume you have an unknown DNA that you know is phage DNA - It is either T7 or lambda. You perform a restriction enzyme digest and run the results on a gel.

  Trend from dominant gametophytes to dominant sporophytes

What trends would they have noticed in Earth's terrestrial vegetation over this period.

  What is the wobble hypothesis

Why are tRNAs referred to as adaptor molecules? What aspects of their structure do tRNAs have in common.

  Cellular respiration and process of photosynthesis of plants

Explaining the cellular respiration and therocess of photosynthesis of plants.

  Find the best experimental combination

Assume you wish to conduct a chi-square test on a set of data obtained from a test cross, to determine whether two genes (A and B) are linked.

  What reward does the male wasp receive from the orchid

Assume you are an ecologist studying the effects of acid precipitation on plant life. Explain an experiment you would perform in order to determine which plant species will be a useful indicator species for acid rain pollution.

  What was the initial concentration of onpg added to tube

A microcentrifuge tube with a final concentration of 0.25 mM ONPG contains 800 uL buffer, 100 uL ONPG, and 100 uL enzyme. What was the initial concentration of ONPG added to this tube?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd