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Question: You are studying the promoter of the rpoA gene in E.coli, which has a -35 consensus sequence of TTGACA. If you mutate the -35 sequence to TTCAGA, will this have any effect on the transcription of the rpoA gene? Why or why not?Question: You create a prokaryotic in vitro transcription system, focusing ot the trp gene in E. coli. To this system, you add all required components for successful transcription in E. coli, plus a compound that adds a third hydrogen bond between all base-paired RNA nucleotides. Will the trp gene be transcribed correctly? Why or why not?Question: If you remove the methyltransferaseenzymes that can add methyl groups to nitrogenous bases, what will happen to mRNA processing in eukaryotes? Why?Question: Construct a eukaryotic in vitro RNA procesing system, focusing on a 23S rRNA in the protist Tetrahymena. To this syste, you add all required components for successful RNA processing in Tetrahymena, except for one of the splieceosome snRNPs. Will the 26S rRNA be processed correctly? Why or why not? Be specific.
he clinical laboratory returns the following arterial blood values for patient: pH = 7.2; plasma HCO3- = 29 mmol/L; plasma pCO2 = 75 mmHg. Determine the patient's acid-base disorder?
Describe the process of the bioremediation in detail. Write down an essay on the topic of bioremediation.
Whether ratios are leverage or profitability ratios. If a leverage ratio, is it coverage or capital structure? What is the dissimilarity between the two? If a profitability ratio, examine why it is not completely satisfactory for measuring an organiz..
Determine which organ system are msot invovled with homeostasis and discuss the role of SNARE in exocytosis.
The molecular weight of "Y" is two hundred. I use 10g of "Y" in 10mL of water. What is the molarity of this solution of "Y"? What percentage solution of "Y" is it?
Which organelle generates oxygen? Which organelle consumes oxygen? Explain why are viruses not considered to be living?
Human population doesn't follow a j-shaped curve since humans are able to consciously limit the number of offspring that they produce, which is what usually happens after a population industrializes.
Determine which intracellular change is the most contributor to increases in fiber size of cross-sectional area and strength?
Who was Carl Weigert? What influence did Weigert have on Ehrlich. Other than the acid-fast stain, what other technique may be used to diagnose tuberculosis? What scientist developed this test.
Give an instance of a successful plant disease forecasting system and explain how it works. How plant diseases could be forecasted and what are the needs for that.
Briefly explain why this mating produces a triploid individual. Why can mitosis proceed normally in triploid cells, but meiosis cannot.
Critically discuss three of the test systems used to study the effects of a toxicant on chromosomes, describe each test system, and discuss one advantage or disadvantage of that particular test system.
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