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The rate of enzymatic denaturation is a strong function of temperature and varies according to the following equation:
Kd = Ad * exp (-Ed/RT) (Ed = deactivation energy, T is in kelvins (K) and R is 2 cal / K-mol)
Increasing the temperature from 40 to 50 C increases kd by 32 fold. What is the value of Ed? Also, if the value of kd at 40C is 0.1 min-1, what is the percentage decrease in enzyme concentration during the enzymatic reaction carried out at 37C for 10 minutes?
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