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A double stranded DNA moelcule with the sequence shown here produces, in vivo, a polypeptide that is five amino acids long.
TACATGATCATTTCACGCAATTTCTAGCATGTAATCTACTAGTAAAGTGCCTTAAAGATCGTACAT
a) Which strand of DNA is transcribed and in which direction? Show your reasoning.
b) Draw the corresponding mRNA sequence and use the genetic code to derive the amino acid seq of the 5 amino acid peptide that is produced.
Discuss how much of a 10X buffer would I add to a reaction whose total volume will be 20?L?
This is the F1 generation in the Mendelian terms. Whether this generation homozygous or heterozygous? What color would its beans be (in other words, what is its phenotype)?
How, at what time the shoot tip touched the other plant's stem did the shoot switch from growing straight to growing in a spiral? Write down a hypothesis first.
The reactions of glycolysis are catalyzed by enzymes and obviously proceed without much problem. However, when we look at some of the individual steps, they have positive standard free energies. What are these steps and how does the process preven..
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The Meselson-Stahl experiment was repeated for a unicellular eukaryote (2N=6) & we learned that DNA replication is semiconservative in eukaryotes.
Mouse L-cells have proven to be an extremely useful model system for investigating the properties of cadherins because they do not normally express any cadherins.
Think about the pathology of a cytokine storm, select possible strategies that would be likely to diminish the harmful effects of superantigen toxins.
What is the most possible explanation. Cultures from dialysate concentrate and the internal areas of the dialysis machines were negative; iodophor from a small in- use plastic container yielded a pure culture of P. aeruginosa. What improper method le..
a.Draw all ionization equilibria for the amino acid cysteine. Label each with the correct pka. b.Calculate the net charge on each structure. c. draw the titration curve d. Calculate the pI.
Describe the steps in performing a karyotype on an adult. Explain how the source of the cells is different in an antenatal testing. Write out the shorthand description of a normal female, normal male, Turner's syndrome, Kleinfelter's syndrome, and..
The European land snail, Cepaea nemoralis, exhibits 3 phenotypes in shell color brown, pink and yellow. Shell color is controlled by one gene that has only two alleles. Brown is dominant to yellow and pink is the heterozygous condition.
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