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Q1. Design pentamer (5 nucleotide) primers that would amplify the entire DNA sequence shown here.
5'-ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3'
3'-TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5'
.
Q2. Draw a restriction map based on the fragments resulting from the following restriction digestions of a 3 kb plasmid
Enzyme Fragment size (kb)
EcoRI 1.0; 2.0
BamHI 1.3; 1.7
EcoRI + BamHI 0.4; 0.6; 0.7; 1.3
They attach to the ring of actin along the cytoplasmic surface of the plasma membrane, causing the actin to contract to form the cleavage furrow.
Consider the following two genotypes: AABB, aabb where A and B are dominant and a and b are recessive.
The rule of independent assortment states that alleles of one gene can segregate independently of alleles of other genes. In other words, the allele you pass on for one trait (for example, eye color) does not have any effect on the allele you pass..
Venison that comes from a deer that is shot before it can run is more tender and not as strongly flavored as venison from a deer that has run to exhaustion. Why?
A human male and female couple with brown eyes discoverthat, in both their families, their fathers (with brown eyes) eachhad siblings with blue eyes.
Simulate a DNA Strand and produce an appropriate transcript RNA molecule for use in translation.
In a cross drosphila involving the x-linked recessive eye mutation white (w) and the autosomally-linked recessive wing mutation curved (c) predict f1 phenotypic results of a cross of true-breeding flies with the phenotypes.
Organ transplant recipients are given immunosuppressive drugs post transplantation. This treatment continues for the rest of their lives to prevent organ rejection.
Explain especially what happens to DCPIP and why and how. Explain results and purposes of each of the four tubes of solutions where the photosynthetic output of the Hill reaction was measured.
Government scientistscontinue to study the bodies of the aliens whose spacecraft crashedin Roswell, NM in 1947. Among other things, the scientists have investigated the structure of the genetic material isolated fromthe aliens' cells
Succinylcholine binds to the ACh receptors at the motor end plate. Explain the mechanism by which it could produce muscle relaxation and why muscle twitches could occur after the administration of succinylcholine. Muscle twitches could occur after..
What range in values would you consider the change to be insignificant for HR, RR and GSR data? Why?
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