Reference no: EM133413372

Question

1. One way to increase the efficiency of the desired DNA ligation product is by having a 1:1 molar ratio between PCR insert to vector. Failing to maintain a 1:1 molar ratio between PCR insert to vector by adding more PCR insert than vector may result in more of which of the following product(s): more than one answer can apply

a) Ligation of a vector with primer-dimers

b) Ligation of a vector with multiple inserts

c) Self-ligation of the PCR insert

d) Self-ligation of the vector

e) Ligation of one PCR insert to a vector

2. Insert to vector ligation reactions are always preceded by a blunting reaction.

a) This statement is false, because all vectors are manipulated to fit the nature of the insert DNA.

b) This statement is false, because ligation creates phosphodiester bonds, which is unrelated to blunting the DNA ends.

c) This statement is true, because blunting reactions prepare the DNA insert to fit the vector.

d) This statement is true, because all DNA ends need to be blunted for ligation to be successful.

e) This statement is false, because as long as both vector and DNA insert have the complementary ends (blunt or sticky) then ligation can occur without a blunting reaction.