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7. A 2 kbp (1 kilo base pairs =1,000 bp) DNA fragment is to be amplified by PCR from the genome of nematode C.elegans. The total genomesize is 105 kbp (108base pairs).
a. Prior to amplification, what fraction of the total DNA does the target sequence constitute?
b. What fraction does it constitute after 10 cycles of PCR?
c. After 25 cycles of PCR?
In general, what is the difference in reaction rate of an enzyme catalyzed reaction in comparison to the rate that the reaction would occur at normally? (I'm looking for a numerical value). Why is this difference observed? Please include a link fo..
Consider brown eyes dominant to blue. A blue-eyed man both of whose parents were brown-eyed marries a brown-eyed woman. They have one child who is blue-eyed. What are the genotypes of all the individuals mentioned?
gene expression is measured by the production of what molecule.
What are the new genotype and allele frequencies for the population? What are the absolute, average, and relative fitnesses for this population as well?
using a volumetric loop is a semiquanitive technique. why is it not quantitative? Design a procedure that would make it quantitative.
Discuss microbiology, and what is one specific positive or negative effect it has had on human health? Be sure to include details about how it has improved or worsened particular aspects of life.
Acetoacetate may be reduced to 3-hydroxybutyrate. What serves as a better reducing agent, NADH or FADH2? Explain.
Most flour beetles are black, but several color variants are known. Crosses of pure-breeding parents created the following results in the F1 generation.
Stress activates neurons in the hypothalamus that eventually lead to the release of glucocorticoids from the adrenal cortex. Describe the neuroscretory pathway that starts with activation of hypothalamic neurons and ends in the adrenal gland.
A double heterozygote has the coupling configuration of A B/ a b of two linked genes that have a frequency of recombination of 0.20. If a randomly chosen gamete carries A, what is the probability that is also carries B?
Assume you are given a test tube containing ten mL of a solution with 6.7 times 109 cells/mL. You are to produce a solution that contains less than 100 cells/mL - but not exactly 100.
If you used the 8900 kilojoules you expend in energy in one day to heat 55000 of water at 24, what would be the rise in temperature?
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