Directhem-agglutination and passive hem-agglutination tests

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What would be the expected results for the directhem-agglutination and passive hem-agglutination tests, which wasperformed as the following?

Two plate:

1.      Add 50 µL of Anti-BSA to A1of the well in both plates.

2.      Add 50 µL of 1X BPS fromA2-A12 and from B1-B11.

3.      Add 50 µL of Anti BSA toA2.

4.      Now, the volume of A2 IS 100µL.

5.      Add 50 µL of BPS to A12.

6.      Add 50 µL of goat/rabbitserum to B12.

7.      Add 50 µL of SRBC to plate #1 from A1-B12.

8.      Add 50 µL of SRBC forCoating & BSA to plate # 2 from A1-B12.

9.      Incubate plates at 4 degreeCelsius overnight and examine on the next day from agglutination.

Reference no: EM13533560

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