Dilution of the entire culture

Assignment Help Biology
Reference no: EM1384071

Question: Assume your professor handed you a test tube with 3.0 ml of an E.coli culture in it and told you to make a 10^(-3) dilution of the entire culture. Describe how you would do it.

Question: ION Relative Permeability [ION]in [ION]out Equilibrium Potential

 

Na+      0.06           10 mM             120 mM         _________ mV

K+         1.0            130 mM             4mM             _________ mV

Cl-        0.90           20 mM            200 mM             _________ mV

Ca++    0.0       1 micrometer        10 mM                __________ mV

a) Approximate the membrane potential (Vm): _____ mV

b) Which ion did you use to get this value and why?

c) What vaue will the Vm reach during the peak of an action potential?

d) Now supply the true, accurate value of Vm using the numbers above:

Reference no: EM1384071

Questions Cloud

Explain use of compensatory substituent in identification : Explain the use of "compensatory substituent" in identification and annotation of non-coding genes? Write down the difference between matrix and a Blosum60 Blosum20 matrix?
Maximum differential settlement under the foundation : Determine the settlements when a stress of 100 kPa is applied by the building over an area of 10m × 5 m on the soil surface. Write a report to show the effect that the sub-layer thickness has on the calculation of the settlement
Estimate the model time series and cross-section : Explain what type of data you would use to estimate the model - time series, cross-section, or panel data and Identify the dependent variable and the independent variables
Management feels that if it does introduce the diet : The Super Cola Company must decide whether or not to introduce a new diet softdrink. Management feels that if it does introduce the diet soda it will yield a profit of $1.25 million
Dilution of the entire culture : Assume your professor handed you a test tube with 3.0 ml of an E.coli culture in it and told you to make a 10^(-3) dilution of the entire culture. Describe how you would do it.
Explain your reasoning why the website has poor usability : From the second e-Activity, explain your reasoning why the Website has poor usability. Propose suggestions on how the company could improve Website usability
Modify solution and categorize it using big-theta notation : Sometimes a slight change in the problem can significantly modify form of its solution. For instance, determine simple algorithm for solving following problem and categorize it using big-theta notation.
Dimensions of the package with largest volume : A package in the shape of a rectangular box can be mailed by the US Postal Service if the sum of its length and girth (the perimeter of a cross-section perpendicular to the length) is at most 108 in. Find the dimensions of the package with largest..
Evaluating strategies on a continuous rather than a periodic : Evaluating strategies on a continuous rather than a periodic basis is desired. Discuss the pros and cons of this statement

Reviews

Write a Review

Biology Questions & Answers

  Creating a simple family tree

In a separate piece of paper, make a simple family tree for a couple showing children, and lineages from two children showing grand children, great grandchildren, great great grandchildren, and great great great grandchildren.

  Define which glucose is transported across a cell membrane

define which glucose is transported across a cell membrane. I could also really use help with finding online references on similar topics.

  Extinction is necessary aspect of evolution

Describe why extinction is necessary aspect of evolution. What would happen if nothing died and there were no extinction?

  Which patient has primary hypercortisolism

Anika's cortisol secretion level falls to normal after the medication, but Bonnie's cortisol secretion remains elevated. Which patient has primary hypercortisolism, also called Cushing's syndrome?

  Explaining specific inheritance pattern

Describe these results by explaining how and why the introns are eliminated and proved the genotypes of Mini, Mickey and Timmy, and their children.

  Double heterozygotes with genotype

Think about the two linked traits in a population in which half of the individuals are double heterozygotes with genotype AB/ab and the other half are double homozygotes.

  Adopted dna as a useful molecule quite independently

He says that the mere presence of DNA is not sufficient to prove the point: his cells may have adopted DNA as a useful molecule quite independently of all other known life forms. What would you do to convince him that he was wrong.

  Normal regulation of blood glucose via pancreatic hormones

Explain normal regulation of blood glucose through the pancreatic hormones. How is this altered with Diabetes?

  How various molecules does one mole of table sugar

In maize, a dominant allele (A) is essential for seed color as opposed to colorless (a). Another gene has a recessive allele (w) which results in waxy starch, as opposed to normal starch (W).

  Expressing a dominant phenotype

A woman expressing a dominant phenotype is heterozygous Dd at the locus. Determine the probability that the dominant allele carried by the woman will be inherited by a grandchild?

  What is the total length of uncut dna molecule

sketch a diagram of the circular DNA molecule, showing the locations of the restriction sites for Ecor I and BamH I , as well as distances between them.

  How to calculate the final dilution factor

Add 1 liter of blood to four liters of water, then take 1 ounce of that solution and add it to 9 ounces of water. at last , take 3 mls of that dilution and add it 567 mls of water.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd