Differences between cloning vectors and expression vectors

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Reference no: EM133285433

Assignment:

1. Using online resources. find coding sequence (gene sequence) of human GLUT1, and identify its protein sequence. Design primers to clone full length of this gene, and design primers to "detect" this gene (embedded PCR).

2. Using PubMed, search for one research article regarding COVID-19 Spike protein STRUCTURE. Search for one MOST RECENT REVIEW article regarding COVID 19 vaccine development. Cite these papers in right format.

3. Both PCR and Elisa are applied in disease diagnosis. Compare the similarities and differences of these two technique in 1) principles of each technique; 2) advantages and limit in their applications in disease diagnosis; 3) give examples of each technique having been applied in clinical diagnosis.

4. The PCR-based diagnosis kit for new COVIC-19 has frequently showed false negative results, discuss what are the possible reasons and how to tackle this problem.

5. The following show the sequence of gene TalB from E. coli, which codes for molybdenum cofactor biosynthesis. Coding sequences of TalB are highlighted. Design a pair of primers to use standard one-step approach to knockout this gene from the genome with selective kanamycin marker.

The conserved sequences for Kanamycin cassette along with conserved Frt sites are:

forward: 5'AAT AAT CAT ACT TAT TCC GGG GAT CCG TCG ACC3'

reverse 5' TGT AGG CTG GAG CTG CTT TCG CAT TGG GGC ATG C 3'

6. When overexpressing a foreign gene in a host system, list some general principles in choosing appropriate expression systems (including choice of expression vector and host). What are differences between cloning vectors and expression vectors? List 2 common promoters in expression vector for E. coli and mammalian cell expression systems.

Reference no: EM133285433

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