Difference between heat fixation and heating the cells

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1. What would the outcome of the gram stain be if you: Forgot to decolorize: Forgot to apply safranin: Left the decolorizer on too long:

2. What is the most important step in the gram stain?

3. Why does the primary stain and the counterstain need to be different colors in the Gram stain?

4. Describe the key difference between Gram + bacteria and Gram - bacteria that contributes to the mechanism of the Gram stain.

5. When would you need to use the acid-fast stain clinically?

6. Why do the spore and acid-fast stains require heat (not heat fixation)?

7. What is the appearance of the acid-fast cells?

8. What is the difference between heat fixation and heating the cells with the stain?

9. How does the appearance of the spores differ from than that of the cell?

Reference no: EM132797342

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