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Consider the cyanobacteria Anabaena. Individual cells grow attached to each other in long chains. most perform photosynthesis using photosystems I and II to generate ATP and NADPH that they use to power the fixation of carbon dioxide via the Calvin cycle. However, a small number form heterocysts, cells that instead of performing photosynthesis, fix nitrogen into ammonium. heterocysts share their nitrogen compounds with their photosynthetic neighbors, who in turn share sugars with the heterocysts.
A) If you extracted all the RNA from a single heterocyst, and all the RNA from a single photosynthetic cell, what information would this give you?
B) Would you find RNA that is the same between these two cell types? If yes suggest two examples?
C) Would you find RNA that is different between these two cell types? if yes suggest two examples?
D) imagine now you used microarray to compare the gene expression of a single heterocyst to a single photosynthetic cell, with the ratio expressed as heterocyst /photosynthetic cell. out of the information for thousands of genes, consider the following three expression ratios , and suggest ( generally based on function you do not need to know the names of the individual genes or proteins produced) what each might be involved in.
gene#1: 23.8
gene#2: 1.002
gene#3: 0.132
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