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The flipping of Phe rings requires that groups surrounding the ring move aside momentarily. The rate of ring flipping can be measured using NMR. You are studying two mutant versions of a protein. In both of the mutants, the proteins have considerable secondary structure, based on circular dichroism studies, and bind the hydrophobic dye ANS, indicating the formation of the hydrophobic core of the protein. However, in one of the mutants (mutant A), the rate of Phe ring flipping is much higher than in the other (mutant B). A researcher suggests that one of these mutants completes the folding process to form a misfolded protein, much like a prion, whereas the other is stuck at some point on the folding pathway.
A. Which mutant forms a misfolded structure? Explain your reasoning.
B. Where along the folding pathway is the other mutant stuck? Again, explain your reasoning.
Reiterate this process of progressively adding the cards over a 15-minute period. Specify if you are able to detect an increasingly dim light longer you spent in dark?
The drug methotrexate has been reported to cause fetal death and/or congenital anomalies, therefore, it is not recommended for women of childbearing potential.
Transformation, conjugation, and transduction were discovered in the laboratory. How important are these mechanisms of genetic recombination in nature.how is electrophoresis used in criminal forensics.
Assuming that genotype essential to produce flat tails is homozygous recessive (such as bb) compute the frequency of the b allele in the squirrel population to two decimal places.
find out the major pathway of glycine degradation. you find that the amino acid serine contains the most radioactivity, and that both the Calpha and Cbeta carbons of the serine residue are labeled with 13C. Explain these observations.
The altered tRNA translates the UGA as tryptophan. What nucleotide varies has probably occurred in the mutant tRNA molecule? What consequences will the presence of such a mutant tRNA have for the translation of the normal genes in this cell.
All blood cells in an adult human can trace their ancestry to which compartment within the body?
Integrative medicine is a way of thinking that applies systems concepts to understand acute and chronic complex disorders. Select a chronic disease such as Diabetes, CVD, obesity or cancer and describe the characteristics of homeostatic system in ..
Two normal parents produce a child with PKU, a recessive genetic disease? Determine the probability that a sperm from the father will contain the PKU allele?
A substitution mutation occurs in the GRE making it unrecongnizable to the glucocorticoid receptor. Determine what impact would this have on transcription of gene downstream of this element?
In Drosophila the genes for wing size and bristle length phenotypes long or short are inherited independently.
compute the isoelectric point of a protein. It would also be helpful if you could declare any constants (i.e. pH=5) in advance of both problems.
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