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You started with a cell count of 9.3 x 105 cells / ml in a total volume of 5.2ml. You need to seed a six-well plate with the following numbers:
2 wells at 1 x 104 cells / well (2ml / well)2 wells at 4 x 104 cells / well (2ml / well)2 wells at 2 x 105 cells / well (2ml / well)
Determine the volume of the suspension you will need to seed one well at each concentration.
Gas gangrene is caused by the highly pathogenic bacterium, Clostridium perfringens. This bacterium destroys animal tissue structure by producing an efficient protease that cleaves sequences of X-gly-pro-Y.
How many dissimilar types of gametes can be produced. how much of drug will stay 6 hours after its administration.
In what way does the fertilization and development of the conifer seed differ from that of the angiosperm?
Recognize the two challenges faced by the public health and also discuss how tools and the guidelines of an epidemiological study is applied in finding the answers and strategies for meeting these challenges.
Discuss how is cancer different from most other types of genetic diseases? Outline Knudson's multistage theory of cancer and explain how it helps to unilateral and bilateral cases of retinoblastoma.
A married couple seeks your advice after having a child affected by an autosomal dominant condition. Studies reveal they are both heterozygous carriers of the mutation but are phenotypically normal.
Do you think that a prenatal test is worthwhile if it detects a genotype, but cannot predict the phenotype? Discuss the risk/benefit issues of this situation.
People say the dose makes the poison, actually, the dose determines the concentration, and the local concentration determines the toxicity the poison.
The diploid genome in a human somatic cell consists of approximately 6 billion base pairs. (a) If you connected all the chromosomes end-to-end, how long is the DNA in each cell based on the crystallographic dimensions of the DNA double helix.
If you were given a sample of chromosomal DNA and asked to determine if it is bacterial or eukaryotic, what experiment would you perform, and what would be your expected results?
ndicate with an arrow the direction that DNA Polymerase would elongate the "primer" DNA strand on this double-stranded DNA molecule in the presence of dNTPs.
How would you interpret this result? How might such a phage replicate its DNA.make sense in terms of Chargaff's rules.
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