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Assume you have isolated a decapeptide (a 10-residue peptide) called "fighting illini (FI)," which has anticancer activity. Determine the sequence of the peptide from the following information. (Note that amino acids are separated by commas when their sequence is not known.). Hint: peptide bonds are not the only type of bonds linking the amino acids to each other in a protein. See insulin as an example.(a) One cycle of Edman degradation of intact FI yields 2 mol of PTH-aspartate per mole of FI.(b) Treatment with ?-mercaptoethanol yields two pentapeptides.(c) Treatment of FI with trypsin in the presence of ?-mercaptoethanol yields three peptides with the following composition: (Ala,Cys,Phe), (Arg,Asp), and (Asp,Cys,Gly,Met,Phe). The intact (Ala,Cys,Phe) peptide yields PTH-cysteine in the first cycle of Edman degradation.(d) Treatment of 1 mol of the intact FI with carboxypeptidase yields 2 mol of phenylalanine.(e) Treatment of the intact pentapeptide (Asp,Cys,Gly,Met,Phe) with CNBr yields two peptides, a dimer and a trimer. The tripeptide yields PTH-glycine in the first round of Edman degradation.
Assume you are studying fruit fly wing development. The wingless gene determines shape of wings. The phenotype associated with dominant allele, W, is normal wing development.
Clarify the principle and advantage of using a rapid identification system. what is the probability that a son born to this woman would be phenotypically normal with respect to both of these traits.
Explain two processes that ensure that your full future sibling is going to be genetically different from you even though you have the same parents.
Prepare 150 microliter of 1X buffer using only water and stock 10X buffer. What volumes of each do you use and change 500 mM EDTA to molarity.
Suppose i f an individual with genotype pQ/Pq heterozygous for both loci in trans arrangement of alleles is crossed with an individual of genotype PQ/pq.
What are the two exceptions to the general rule that all cells in the human body contain a complete, identical copy, of a person's DNA?
Suppose the gene for dimples, d, is recessive. Then suppose that two individuals with the genotypes Dd and dd mate.
Which of these statements can apply to endocrine disruptors.
Achondroplasia is caused by an autosomal dominant mutation within the fibroblast growth factor receptor-3 gene on the short arm of chromosome four.
What disorder does this man have? Gastric secretions normally include about 10mmole/L potassium. How do you account for the low serum potassium in this patient.
If I had 1L of a 1.0M solution of glycine at the isoelectric pH (6.02) and I added 0.3 mol of HCL,explain What would be the pH.
A botanist takes a sample of 300 plants from a population and determines the genotype of each one for an esterase locus. The locus has two alleles segregating in the population
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