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Question: A linear fragment of DNA that is 22 Kb is cleaved with the individual restriction enzymes SalI, HindIII and KpnI and then in the pair wise combinations listed below. The sizes of DNA fragments seen on the resulting ethidium bromide stained gel are:SalI: 11 Kb, 6 Kb, 5 KbHindIII: 14 Kb, 8 KbKpnI: 16 Kb, 6 KbSalI and HindIII: 8 Kb, 6Kb, 5Kb, 3KbSalI and KpnI: 11 Kb, 5 Kb, 1 KbHindIII and KpnI: 8 Kb, 6 KbDetermine the restriction map for the linear DNA fragment (ie: figure out where each restriction enzymes' cut sites are located on the DNA fragment).Select the correct order in which the restriction enzyme cut sites would occur on the linear DNA fragment beginning at one end of the fragment.Select one:a. KpnI, SalI, SalI, HindIIIb. KpnI, SalI, HindIII, SalIc. HindIII, SalI, KpnI, SalId. KpnI, SalI, HindIII
The global community is plagued by increasing incidence of lung cancer, colorectal cancer, breast and pancreatic cancers, prostate cancer, leukemia, non-Hodgkin lymphoma, liver, ovarian and esophageal cancers.
Chimpanzees have 24-pairs of chromosomes. Chimpanzee somatic cells contain DNA double helices.
Assume your supervisor has found a new test kit that she wants to start using. She has asked you to validate the kit. Please explain some tests that might be needed?
Suppose that the long ear lobes in humans is an autosomal dominant trait that exhibits 30 percent penetrance. A person who is heterozygous for long ear lobes mates with a person who is homozygous for normal ear lobes.
The human body is truly remarkable and is designed to function effectively. Organs are important structures composed of different tissues that facilitate specific functions within the body.
Determine how to calculate osmolality for an electrolyte versus nonelectrolytes and molality versus osmolality.
An assured drug that is used to control high blood pressure stimulates K+ excretion in the kidney such that plasma [K+] decreases from a normal value of 5 mM to 2.5 mM. Intracellular [K+] is largely unaffected. What effect this drug will have on the ..
Discuss what determines the shape of fungal spores that are ejected into the air? Develop an hypothesis and suggest an experiment
Assume y ou wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volume.
Some sensory neurons in our bodies generate action potentials in response to pressure or movement, allowing us to feel things through our sense of touch.
Assuming that the virus capsid is spherical, work with your classmates to calculate what is the maximum genome size (in nucleotides) that can fit into the capsid. How long in millimeters would this largest possible RNA genome be?
find if your unknown organism ferments mannitol. What are the two tests?
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