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You have identified a mutant E. coli strain that cannot synthesize histidine (His-).To determine the location of the his- mutation on the E. coli chromosome, interrupted mating experiment was performed with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His- strain.
Hfr A -------- his (1) man (9) gal (28) lac (37) thr (45)Hfr B -------- man(15) his (23) cys (38) ser (42) arg (49)Hfr C -------- thr (3) lac (11) gal (20) man (39) his (47)Hfr D -------- cys (3) his (18) man (26) gal (45) lac (54)Hfr E -------- thr (6) rha (18) arg (36) ser (43) cys (47)
On the map below of the circular E. coli chromosome, indicate:
a. the relative location of each gene
b. the position where the sex factor is integrated in each of the five Hfr strains
c. the direction of chromosome transfer for each Hfr (indicate direction with an arrow).
You run a set of reactions to determine the reaction rate as a function of substrate concentration and notice that the rate decreases as your substrate concentration increases.
You are working with an enzyme that catalyzes the deamidation of Gln residues in proteins. You have developed two different tripeptide substrates for the enzyme.
Explain each of the stages of viral reproduction: Absorption Penetration Synthesis Maturation Release
If iodine were not available, how would you determine whether starch hydrolysis had occurred? Some may see starch agar plate as an answer, however, isn't that already being used to grow the bacteria?
In a germination experiment the germination rate and timing of wild radish (Raphanus raphanistrum) and crop radish (Raphanus sativus) were measured.
A miRNA regulates its target mRNA through degradation. Let us suppose miRNA-199 targets Blimp1. Which progenitor population in the developing embryo will be affected?
Which of the following statements regarding Nucleotide Excision Repair (NER) and Base Excision Repair (BER) is true.
The term "hominid" has traditionally been used to refer to species in the line the leads to modern humans after the divergence between that line and the line that leads to modern chimpanzees.
what are tow advantages of using genetic engineering to produce human proteins to treat disorders instead of animal or human sources.
Every cell in the human body has two alleles that condense into single chromosomes held together by a centromere. These "sister" chromaatids replicate and pair with the newly made homologous chromosomes.
Is it possible for one kind of neurotransmitter molecule to have an excitatory effect at one cell, and an inhibitory effect at another? Explain using an example.
A rather tame ltxnsp was recently found on Easter Island through a retired neurophysiologist. He reported the following measurements of ECF and ICF concentrations
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