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You have successfully developed two new inhibitors (Alice-1 & Alice-2) for the mutant peptidase mentioned in question To understand how the inhibitors work, you carried out enzyme assays measuring the ability of the mutant peptidase to cleave the substrate in the absence and presence of the two inhibitors and obtained following data:
Using the entire data set to make a diagnostic plot, answer the following:
V0, ?M/sec
[S], ?M
No inhibitor
+ Alice-1 (10 nM)
+ Alice-2 (10 nM)
2
0.062
0.006
0.042
4
0.118
0.012
0.080
8
0.210
0.024
0.157
16
0.348
0.045
0.256
(a) Does Alice-1 or Alice-2 greatly affect the ability of the mutant peptidase to carry out the cleavage reaction (kcat)?
(b) Determine the Km of the mutant proteases for its substrate in the absence and presence of inhibitors.
(c) Which inhibitor is more effective?(d) What types of inhibitors are Alice-1 and Alice-2?
Use the momentum balance method and derive the flow rate of a Newtonian fluid flowing across a cylindrical tube driven under pressure of rate change if the tube diameter decreases by half?
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Perform visual comparison of the assigned two putatively related nucleic acid sequences x and y. Indicate in your answer the identities by 1s on match and 0s on mismatches. Also indicate mutations by the scoring indices S for transition and V for ..
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Arrange the amino acid substitutions in increasing order of conservatism and amino acids are given using one letter codes to give you some practice in using those.
how The information carried by DNA is incorporated in a code
Assume you have 5mL of cells you need to treat with Hydrogen Peroxide, such that the final concentration of Hydrogen Peroxide is 5uM. How much of a 10mM stock solution of Hydrogen Peroxide would you add to your cells?
Determine the principal reaction that makes an organic, nitrogen containing molecule, capturing the nitrogen in ammonia created through nitrogen fixation.
Dominant-recessive relationships can make determining genotype tricky, since an individual with the dominant phenotype may have either the homozygous dominant genotype or the heterozygous phenotype.
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