Determine the function of protein

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You have recently isolated the cDNA of a gene from mouse lymphoid tissue. So far, you've sequenced the ORF and analyzed the predicted protein product by comparison with the protein data basis available and your protein appears to contain a DNA binding motif. From the database comparison you have done, this motif shares significant homology with other lymphoid transcription factors known to bind DNA in a sequence specific manner & enhance/regulate the transcription of a specific class (Class G) of lymphatic tissue genes. You wish to determine the function of your protein. Outline the steps of the procedures you might use to determine its function.

Reference no: EM13145631

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