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1) Assume that you measure the OD of an overnight E.coli culture. The spectrophotometer gives you a reading of 2.8. What would you do to determine the concentration of your overnight culture and why?
2) The OD of an E.coli culture is 0.23. You transfer 300 µl of this culture into 29.7ml of medium. You continue this same dilution one more time. You transfer 50 µl of your last dilution onto an LA plate. After 24 hours of incubation, you observe 11 colonies on this last plate. A) Did you expect this result? If not, name at least three reasons for this result.
3) A friend of yours asks you to help him/her draw a bacterial growth curve. He/she gives you the following information: the TA started a fresh culture at 8:30am (the OD of this fresh culture was 0.14). During class the following OD readings were obtained: 0.19 at 9:50am, 0.28 at 10:10am, 0.29 at 10:30, 0.68 at 10:50 am, and 1.1 at 11:30am. At 11:30 am the OD of a 1:10 dilution of the stock culture was 0.29. A) Draw the graph. B) What was the generation time of this culture?
4) For a different experiment you are growing up Saccharomyces cerivisiae instead of E.coli. The measured OD is 0.89. A) In order to calculate the concentration of the Saccharomyces cerivisiae culture, can you use the equation that you use to calculate the concentration of an E.coli culture? If yes or no, give a detailed explanation. B) Does E.coli or Saccharomyces cerivisiae have a shorter generation time?
How might you deterine whether the turbidity in your nutrient broth tube was from a mixture of different microbes or from the growth of only one kind of microbe.
Which of the following is not a consequence of genetic drift.
When William was helping victims following a devastating earthquake in a region that was not prepared to swiftly set up adequate temporary shelter,
What is the molarity of the drug in he Petri plates?
A sunflower population in Hardy-Weinberg equilibrium has the given allele frequencies for petal color: 0.75 dominant, 0.25 recessive.
Describe the difference between isotonic and isometric contractions. What differences did you notice between the spongy and compact bone?
Gases move from areas of high (total) gas pressure to areas of low (total) gas pressure. Also, the pressure of a "canned" gas changes if the container volume changes.
Assume that a man with dentinogenesis imperfecta, whose father had the disease but whose mother had normal teeth, married a woman with normal teeth. They have six children.What is the probability that this couple's first child will be a male with ..
Draw the synaptic configuration of homologous pairs of chromosomes when one member is normal and the other member has sustained a duplication within the chromosome
select a chromosomal disorder from the March of Dimes Foundation Website.
How does sweating help you regulate your body temperature? Why do you feel hotter and more uncomfortable on a hot, humid day than on a hot, dry day?
Determine what type of information is provided by the RBC indices and discuss h ow statistically relevant is this information?
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