Determine how many genes are located in the rii region

Reference no: EM13332676

Mutations in the rII region of a bacteriophage T4 prevent its growth on E. coli strain K. You obtain numerous mutations in this region and want to determine how many genes are located there. You perform double infections of E. coli K with the different pairwise combinations of mutations both in cis and in trans-configuration. (Recall that cis-configuration means that two mutations are located in the same phage genome, and trans means that they are located in different genomes).

Cis-test Trans-test
rIIA rIIB / + + ==> growth rIIA + / + rIIB ==> growth
rIIA rIIC / + + ==> growth rIIA + / + rIIC ==> growth
rIIB rIIC / + + ==> growth rIIB + / + rIIC ==> no growth
rIIA rIID / + + ==> no growth rIIA + / + rIID ==> no growth

What can be concluded from this experiment?

Reference no: EM13332676

Questions Cloud

Find the angular acceleration of the pulley : A 2.93kg stone is tied to a thin, light wire wrapped around the outer edge of the uniform 9.83kg cylindrical pulley, Find the angular acceleration of the pulley

Explain what are the concentrations of a and b : A solution containing both A and B in a 1-cm cell showed an absorbance of 0.726 at 267 nm and 0.544 at 312 nm. What are the concentrations, mg/L, of A and B

Determine what are the genotypes of the parents : a mating of an albino (White) guinea pig and a black one gave 6 borwn, 3 black and 3 white offspring in the f2 generation. what were the genotypes of the albino (Cw) and the black (Cb) parent guinea pig respectively

What is the harrys velocity : Harry Potter (55kg) is riding his broom north when a Slytherin casts a spell to knock him from the broom. what is Harry's velocity

Determine how many genes are located in the rii region : Mutations in the rII region of a bacteriophage T4 prevent its growth on E. coli strain K. You obtain numerous mutations in this region and want to determine how many genes are located there.

Imposition of quotas on foreign steel sold in the us : Why might U.S. steel-using firms lobby against the imposition of quotas on foreign steel sold in the United States?

Explain calculate what the slope of the boundary : Balance the following half reaction: HS(-) = S2O3(2-) AND calculate what the slope of the boundary between HS- and S2O3^2- is in a pe-pH diagram.

How fast is the ubg traveling after the collision : A prehistoic bug (m=0.02kg) has escaped into the moder world. how fast (and in what direction) is the ubg traveling after the collision

Explain which orbitals on the chlorine and iodine atoms : Which orbitals on the chlorine and iodine atoms overlap in the formation of the covalent bond in I-Cl

User Account

All Pages