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You decide to design an assay for flu infection detection. HA protein is only present on the surface of influenza viral particles so you choose to design an ELISA assay to detect the presence of HA in human blood samples. You will use THREE different antibodies for the assay. The experimental procedure is as follows: Step 1. Incubate your assay plate with antibody (I) solution overnight to coat the surface of the assay plate with antibody (I). Remove antibody (I) solution and wash the plate extensively. Step 2. Add blood samples to the coated plate and incubate for 30 min. Remove blood sample and wash the plate extensively. Step 3. Add antibody (II) solution to the plate and incubate for 30 min. Remove antibody (II) solution and wash the plate extensively. Step 4. Add antibody (III) solution to the plate and incubate for 30 min. Remove antibody (III) solution and wash the plate extensively. Step 5. Add TMB substrate to the plate and monitor color development. In your lab, you have the following reagents on hand: Rabbit anti-HA polyclonal antibody Mouse anti-HA monoclonal antibody Rabbit anti-HA monoclonal antibody Goat anti-rabbit polyclonal antibody conjugated to HRP Goat anti-mouse polyclonal antibody conjugated to HRP You decide to use Rabbit anti-HA polyclonal antibody to coat the surface of your assay plate. What would be an appropriate negative control sample for your ELISA assay? (1pt) What is the expected colorimetric result from the negative control in your assay? (2pt) What would be an appropriate positive control sample for your ELISA assay? (1pt) What is the expected colorimetric result from the positive control? (2pt) Indicate whether or not the following antibody combinations could be used to successfully perform your assay. If a pair can't be used successfully, indicate why not. (6pts) ? Rabbit anti-HA polyclonal antibody as antibody (II) and Goat anti-rabbit polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no ? Mouse anti-HA monoclonal antibody as antibody (II) and Goat anti-rabbit polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no ? Mouse anti-HA monoclonal antibody as antibody (II) and Goat anti-mouse polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no
Chemical reactions obey a relationship between reaction ratek and activation energy ΔG‡ that canbe written k =Qexp(-ΔG‡/RT) where Q is a constant characteristic of the reaction,R is the gas constant, 0.001986 kcal/(mol-deg), andT is the Kelvin t..
Tasmanian devils once inhabited most of present day Australia, but only an isolated population on the island of Tasmania has survived to present day. What process has likely affected Tasmanian devils as a result of this history
Was the urine culture set up correctly and Does blood agar support the growth of both gram-positive and gram-negative organisms?
In wild petunias, the allele for red flower color ( R ) is dominant over the allele for white flower color ( r ). We sample a population and find 225 plants have white flowers and seventy-five have red flowers.
Cystic fibrosis is a genetic disease that leads to formation of thickened mucus in the lungs, pancrease, and other organs. In the lungs, the mucus impeads airflow and the removal of particulates leading to infection.
The UV absorption in proteins at 280 nm is largely due to tryptophan residues. The molar extinction coefficient of tryptophan is 5380 M-1cm-1. Exactly 0.25 g of a protein is dissolved in 200 milliliters of a neutral buffer.
why DNA was discarded as a possible candiate
Post-translational modifications of proteins play crucial regulatory functions. Identify three different post-translational modifications and describe two different techniques that would distinguish a modified versus unmodified form of the same pr..
0.00100 mol of HCl, a strong acid is dissolved in 100.0L of water, What is the molarity of the acid and what is the [H+] ions
Which animals have four-chambered hearts? The barrier to invasion by microbes involves?. Which are divided into two groups: T cells and B cells? Macrophages, lymphocytes, complete cells, or platelets
1. Compare the embryos of chickens, humans and fish, and describe in detail how pharyngeal arches show a shared evolutionary past?
Surgery of the neck results in damage to a cranial nerve. The result is that the patient experiences a decreased ability to alter breathing pattern in response to low blood oxygen levels. Which two cranial nerves could cause this id famaged?
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