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You have purified a protein, MT1, that binds only to GTP-bound tubulin. You proceed to fuse MT1 to GFP and find that the fusion of GFP to MT1 does not disrupt MT1 function in any way (good news!). You use a different dye to label the microtubules in red so that you can visualize MT1-GFP and the microtubules at the same time. (Note that the red dye does not affect the microtubule's properties.)
A. If you were to polymerize some red-labeled microtubules in a dish, would you expect MT1-GFP to be associated with one or both ends of a microtubule, with the middle of the microtubule, or be evenly distributed throughout the microtubule? Explain.
B. If you were to take time-lapse images in which you visualize only MT1- GFP (and not microtubules), what would you expect to see? Describe what you think would happen to MT1-GFP over time with respect to its localization inside of this cell.
The time-of-entry mapping method was used to determine the first five markers transferred from each Hfr during a synchronized conjugation experiment. The markers and their transfer order form each Hfr are shown below.
once lodgepole pines are established the forest of yellowstone park will not change radically again.what is the term for the lodgepole pine community?
How may map units separate e and pe? To calculate the map units separating the genes, count the number of recombinant offspring and express this figure as a percentage of the total progeny.
Explain each of the stages of viral reproduction: Absorption Penetration Synthesis Maturation Release
Southern Gas recently paid a $2.75 annual dividend on its common stock. This dividend increases at an average rate of 4% per year. The stock is currently selling for $27.50 a share. What is the market rate of return?
Leon hopes to become an ichthyologist (fish biologist). What category of organisms does Leon hope to study?
If the mutation in the Saccharomyces cerevisiae is an ochre mutation (see introduction), what amino acids may be substituted into the polypeptide by a single base mutation?
All cells (including prokaryotic cells) undergo cellularrespiration in order to produce energy. Cyanobacteria are bacteriathat undergo photosynthesis.
What is one characteristic that some archaea possess that bacteria and eukarya do not?
Assume you incubated 10ml of a solution containing 0.15M G6P overnight at 25 degree C with the enzyme phophoglucose isomerase. How many millimoles of F6P will be in the 10ml solution in the next morning.
Describe the mechanics of inspiration and expiration.
What can be intellectually stimulating or knowledgeable that can be gained from the study of Microbiology?
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