Describe what materials are required in pcr reaction

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Reference no: EM133932532

Questions

1. Describe what materials are required in a PCR reaction.

2. What is primer dimer in a PCR reaction? How do you avoid primer dimers when designing PCR primers

1. What is the effect of the following agents on DNA?

a) Ethidium Bromide
b) Ultraviolet light
c) Nitrous acid
d) Acridine Dyes

2. What are base analogues - give examples.

3. If AUG codes amino acid Methionine- then if there are transition mutations happening on this code what all amino acids can be generated? Take help of Genetic Code Table

4. If Amino acid GAG codes for Glutamic Acid, then if transversion mutation occurs what all amino acids will be generated.

5. If an mRNA strands read like 5' -AUG GCA CAG ACG UGA-3" then what amino acids will this mRNA code for? i) add a Base "G" after the code GCA and then write which amino acids will be generated; ii) add GC after the code GCA and the write which amino acid will coded; iii) aa GCG after GCA and then tell which amino acids will be coded by this mRNA.

6. If an mRNA strands read like 5'-AUG AGG CGA GCA UAG-3' then which amino acid will be coded.

i) What will happen in nucleotide "A" is removed from 4 th position.
ii) What will happen in nucleotide "A" and "G" are removed from 4 th and 5th positions.
iii) What will happen in nucleotide "A" and "G" and "G" are removed from 4 th, 5 th and 6th positions.

Reference no: EM133932532

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