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Describe the mechanism of ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the microtiter wells? Why is it important to have a positive control?
List and describe the five steps required to visualize a bacterium successfully under an electron microscope.
Determine which of the following statements is accurate regarding the evolution of drug resistance in a virus?
Sort the statements into appropriate bins depending on which type of learning they explain. What is her anatomical dead space volume.
Represent graphically the relation between melting temperature and the G-C content of DNA. Construct relations for dependence of melting temperature of a 30 bp DNA duplex on the G-C content.
Assume you were given fifty ml of sewage and asked to determine the number of bacteria in the sample. You began by taking five ml and putting it into 45 ml of sterile water.
Say you injected and x amount of protein onto SEC column and it produced certain peaks. Now say you repeated the experiment and use the same amount of protein.
Explain why dihybird crosses of yeast always results in predicted phenotypic ratio but results of similar two crosses in eukaryotic organisms
Describe the specific structural elements and factors involved in the synthesis of a transmembrane protein in contrast to a cytosolic or nuclear protein.
Assume you identify a recessive mutation in the AP endonuclease in yeast. During the diploid phase of their lifecycle, the yeast are viable but haploid strains die rapidly.
What is the function of the vitellus in the vertebrate egg? How are these eggs classified according to the amount of vitellus within them?
You have a 10x enzyme buffer that you are to use in a particular reaction. The total volume of the reaction is 30 micro liters. Determine how much of the 10x buffer should you add so that the final concentration of buffer in the reaction is 1x?
At a criminal trial, the prosecutor presents genotypes for three microsatellite loci as evidence. She reports that a DNA sample taken from the crime scene.
Would two nucleotides at a time be sufficient to provide enough codons to code for all twenty amino acids? Discuss how many amino acids could be coded for by codons containing only two nucleotides?
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