Describe the mechanism of action of a suicide vector

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Question 1. Your coworker and good friend has just returned from a trip abroad (since he works for the CIA, he can't tell you where he has been). Two days after his return, he falls sick with a severe infection. Your job as his friend is to find the best (and most efficient) way to identify what caused the sickness? What molecular diagnostic approach will you use? Once you have identifies the cause, what treatment will you develop and why and how will you do it so your friend is quickly back to normal?

Question 2. After a PCR run using a new set of primers, upon electrophoresis, your gel shows no amplification bands at all. Suggest three reasons with explanation which could lead to such a result?

Question 3. Describe the mechanism of action of a suicide vector - why is it needed, how does it work, why does it have this name?

Question 4. Describe what is meant by a "cell free" method used to produce protein arrays. Identify two such methods and explain why they might be advantageous compares to cellbased technologies?

Reference no: EM133311117

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