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1) I have to find the total yield of protein (rGFP) given estimated purity (in %) and amount of proteins. I have the purity of the protein and the amount of protein, so how do I find the total yeild from these values.
2) The expression and purification of rGFP started with the growth of the bacterial culture and ended with elution of rGFP off the Ni+2 agarose coloumn. Describe one change to this process that you predict would result in an increase of the total yield of rGFP.
In modern blimps, the gas of choice used to inflate them is helium rather than hydrogen. Hydrogen would be lighter, but helium is safer. Compare and contrast the atomic structure of hydrogen and helium.
Is this organism recovered more frequently as a nosocomial pathogen, as a community-acquired pathogen, or at similar rates in both settings? Elucidate your choice.
Suppose that red reen color blindness is a recessive sex linked trait. A normal man mates with a color blind woman, what can we expect their children to be? Explain your answer.
Assume you are analyzing microbial life in the Rocky Mountains. You scoop up a water sample from a melted snow puddle because you are curious about the possible microbes that might live there.
Assume you are studying metabolic pathways and discover that 2-pathways intersect so that enzyme basinase participates in both of the intersecting pathways.
Which extinct species should be the best candidate to serve as the out-group for clade whose common ancestor occurs at position 2.
Noah is a technician assigned to photograph chromosomes. At what stage of the cell cycle are the chromosomes sufficiently condensed that they FIRST become visible under light microscope.
Swelling of the abdomen and legs due to edema is characteristic of starving children with kwashiorkor, protein-deficiency disorder. What is the relationship between this symptom and procedure of capillary exchange.
Why were the ratios the similar in the first part but different in Part 2.
What disorder does this man have? Gastric secretions normally include about 10mmole/L potassium. How do you account for the low serum potassium in this patient.
The internal structure of the cells of many protists is much more complex than cells of multicellular organisms. Does this mean that protists is engaged in more complex activities than muticellular organisms?
Which of the following statements is true? The first cervical vertebra is called the axis.The spinal cord protects the vertebral column.
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