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The synthesis of a phosphodiester bond involves condensation of a5'- phosphoryl group on one nucleotide with a 3' hydroxyl group onan adjacent nucleotide. DNA Ligases catalyze this reaction bycoupling condensation to cleavage of a phosphoanhydride bond fromeither NAD+ or ATP.
The overall reaction catalyzed by T4 DNA ligase is seen inthis equation: ATP + nicked bond -> AMP + PPi +phosphodiester bond
This is actually the sum of two half reactions. The sum of a hydrolysis reaction coupled to a condensation reaction is thermodynamically equivalent to the ligase reaction represented by the overall reaction (above).
What are the two half reactions?
A couple is about to have a child. They are concerned about the child because of two traits caused by genes on the SAME chromosome.
Which of the following is not true of spermatogenesis?
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how such a mutation might alter the expression of a gene.
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A different walnut rooster was crossed to a rose hen, and all the progeny were walnut. What are the probable genotypes of the parents.
What is the differences in transport systems of unicellular protists, colonial protists and large multicellular protists, such as brown algae.
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