Define the term scale up, Define the term scale up. Explain

Define the term scale up

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Reference no: EM134000266

Assignment:

EXERCISE- ANIMAL CELL CULTURE TECHNOLOGY

1. Define the term scale up.

2. Explain the 2 approaches for animal cell culture scale up

3. In your opinion, can we use the same bioreactor for microbes and animal cells? Justify your answer with THREE reasons.

4. If you were assigned the job to buy a bioreactor for animal cell culture to be used in your lab, explain FOUR requirements that the bioreactor that you choose should fulfil.

5. Draw a simple structure of STR with glass design and label its components.

6. Sharmini is culturing her animal cells in a bioreactor. However, she often have a problem of pH fluctuations. Suggest how she can overcome this problem.

7. If you are using a cylindrical shape bioreactor, show how to compute the aspect ratio.

8. a) Draw a simple diagram of spinner flasks.

b) Tabulate 3 advantages and disadvantages of scale-up monolayer approach

9. List down the parameters that need to be controlled/ monitored while doing cell culture in bioreactor.

10. Explain why the agitation for microcarrier cultures should be lower compared to suspension cultures.

Reference no: EM134000266

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