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A kan-r, tet-r plasmid is treated with the restriction enzymeBglI, which cleaves the kan (kanamycin) gene. The DNA is annealed and ligated to a BglI digest of Neurospora DNA and thenused to transform E. coli.
a. What anitbiotic would you put in the growth medium to ensure that each colony has the plasmid?
b. What antibiotic-resistance phenotypes will be foundamong the resulting colonies?
c. Which phenotype will contain Neurospora DNA inserts?
What methods would be used to determine the arrangement of atoms within a protein? This method has also been used to detect and locate the spatial arrangement of the atoms of DNA.
He observed that all tissues except the CNS/brain turned blue. Based on what you know about the CNS, explain Ehrlich's results. What experiment could you design in order to stain the brain a blue color?
The binomial name of an organism consists of the names of which two classification categories. Smoking, air pollution and altitude all affect the rate of respiration. Explain the effects of each of these on respiration and Why.
A mammal uses only one to two percent of its energy in ventilation as a fish must spend about 15 percent of its energy to move water over its gills. Explain this huge difference in their efforts to collect oxygen.
In modern blimps, the gas of choice used to inflate them is helium rather than hydrogen. Hydrogen would be lighter, but helium is safer. Discuss similarities and differences of the atomic structure of hydrogen and helium.
Describe why population growth in the human population does not seem to be affected by limiting factors and what are the effects of sympathetic nervous system on the glomerular filtration rate (GFR)?
based on prevalence numbers i need to find how many ppl in acollege (2514 ppl) have cystic fibrosis. what do i do to find howmany are carriers of cystic fibrosis with these stats
You are involved in a biodiversity survey and encounter a meadow of grass. How would you best describe the grass?
Which of the following is TRUE of fungi?
What is the geochemical impact of the heterotrophic microbial activity in freshwater habitats?
What is the difference between a t-test and a chi-square test? How are the results different.
A gerbil poplulation has one hundred light blue gerbiles, 250 dark blue gerbils, and 150 black gerbils. In one generation 10 percent of the home population is displaced by a gang of tough light blue gerbils.
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