Define restriction fragment length polymorphism

Assignment Help Biology
Reference no: EM131366426

1. Severe combined immunodeficiency disease (SCID) is fatal at an early age. SCID is caused by the lack of the enzyme adenosine deaminase, which is encoded by the gene ADA. Suppose you want to use a gene therapy method to treat the disease. You have DNA that contains the ADA gene and a virus that infects human cells. Design a protocol to get the ADA gene into the viral DNA.

2. Define restriction fragment length polymorphism (RFLP) analysis. The information generated from RFLP is useful in criminal investigations. Suppose some DNA samples are taken from a crime scene, suspect, and the victim. The samples are amplified by PCR, and then subjected to RFLP analysis. Below is a gel electrophoresis of the RFLP results. D1 is from defendant 1; D2 is from defendant 2; V is from the victim; and S1, S2, and S3 are from the crime scene. Speculate on how these results would have an impact on each defendant.

3. Define genomics and proteomics. Name two techniques that are used in each field of study. Protein is translated from mRNA, which is transcribed from the gene. However, the proteome is much larger than the genome for a given organism. Give two reasons why there are more proteins than type of genes.

Reference no: EM131366426

Questions Cloud

Discuss the events and methods of production : Meal analysis: Discuss the events and methods of production that allow major grocery chain stores to carry these items. Source identification: If you were to purchase each item at a major grocery chain store, where would these items be sourced
Business communication essentials : Select a product or service for this assignment in which you believe your organization should invest capital resources to develop for sale in a global environment. Review Ch. 4 of Business Communication Essentials.
Identify which leaders were involved in the communication : Explain how change was communicated. Identify which leaders were involved in the communication. Identify change model.Format your assignment consistent with APA guidelines, with a reference page and corresponding in-text citations.
Give the answer of muliple choice question : Historical research is chiefly concerned with:To a historical researcher studying the lives of women pioneers in the late 1880s, letters written by Rebecca Adams, a pioneer woman on the Nebraska plains, to her sister in Philadelphia would be:
Define restriction fragment length polymorphism : Define restriction fragment length polymorphism (RFLP) analysis. The information generated from RFLP is useful in criminal investigations. Suppose some DNA samples are taken from a crime scene, suspect, and the victim. The samples are amplified..
What interested you about the topic : Provide a brief summary of the article. What interested you about the topic? Do you agree or support the discovery or activity presented in your article? Why or why not
Display characteristics displayed by your companys abilities : Display the characteristics displayed by your company's abilities as a learning organization. Explain how your company's culture and learning organizational abilities may support or detract from a change initiative.
Define polymerase chain reaction : 1. Define polymerase chain reaction (PCR). Demonstrate one cycle of the PCR process starting with one piece of DNA fragment. In the drawing, label template DNA, primers, dNTPs, and DNA polymerase.
Where does the essay seem sparse : Is 'the progression of ideas in the essay clear? Have the writer go over it with you - What is the conclusion? Does it seem to be based on the material? (Don't just answer 'yes' here)

Reviews

Write a Review

Biology Questions & Answers

  Determine the position of a nucleotides in dna

If you need to determine the position of A nucleotides in DNA using dideoxysequencing, you need to include a primer and ddATP in addition to 4 dNTPs for DNA extension. If you need to detect as long of a sequence as possible, you should include a r..

  Solution of glucose

How would you make a 0.1 M solution of glucose The massnumbers for these elements are C=12 O=16 H=1.

  Depolarization of the ventricles

Depolarization of the atria is followed by depolarization of the ventricles. Given that the mass of the ventricles is much greater than the mass of the atria, which of the following ismostly likely to be true?

  Develop the rudiments of a maintenance diet

You probably want to live a long and healthy life on this earth. What are you willing to do to make that possible? Here is an assignment that can improve the quality of what you eat, and hence, the quality of your life. Let us develop the rudiments o..

  How might the discovery of a gene benefit from a patent

Researchers working on the Human Genome Project have determined the nucleotide sequences of human genes and in many cases identified the proteins encoded by the genes. How might the discovery of a gene benefit from a patent

  Explain how some aspect of skin cells demonstrate

Explain how some aspect of skin cells demonstrate form follows function.

  How can we be certain that growth with a metallic sheen

In the diversity and abundance of bacteria, we always have some uncertainty about the identity of an isolate. With this in mind, how can we be certain that growth with a metallic sheen is truly a coliform bacterium

  How long would the channels need to stay open

Cytosolic Ca2+ concentrations typically rise about 50-fold when Ca2+ channels open in the plasma membrane. Assume that 1000 Ca2+ channels open in a cell with a volume of 1000 µm3 and an internal Ca2+ concentration of 100 nM.

  Find how many autosomes are present in mouse gamete

There are forty chromosomes in somatic cells of the house mouse. how many chromosomes does a mouse receive from its father and how many autosomes are present in a mouse gamete?

  Discuss what a prepared organization is to kaufman

Discuss what a prepared organization is to Kaufman. Do you believe this is what it will take to effectively deal with the health care bubble? What are some suggestions that you have based upon this article and your own research?

  What is probability that first child will be a normal girl

Phenylketonuria is an inherited disease caused by a recessive autosomal allele. If a woman and her husband are both carriers, what is the probability that their first child will be a phenotypically normal girl

  How can electrolyte imbalance control our heart function

Electrolyte balance is very important in our body. This balance can even greatly influence our heart functions - causing arrhythmia or even a heart attack. Lets discuss this situation - how can electrolyte imbalance control our heart function?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd