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1. You are given a milk sample that Mr. Milkman claims contains 4.5 x 108 CFU/ml. He claims this is within the limit of acceptable CFU/ml for Grade A milk. Diagram a procedure he could use to determine the CFU/ml and information pertinent to proving or disproving Mr. Milkman's claim. Is the milk within the acceptable limit?2. A plate of nutrient agar, labeled 10-4, has 255 colonies on it and another plate of media, labeled 10-5, has 27 colonies on it. What would be the number of CFU/ml in the original sample?3. I give you 6 mls of an overnight culture of Micrococcus luteus at a concentration of 4.5 x 106 CFU/ml. Diagram a dilution series to obtain countable plates by using tubes containing 9 ml nutrient broth (NB) and fixed-volume 1ml pipettes (won't measure any more or less than 1ml).4. I give you a very expensive strain of E. coli containing 106 CFU/ml and tell you that I need exactly 10ml of the same E. coli culture at a concentration of 103 CFU/ml (I don't want any excess culture). Using a dilution series, how would you get the correct concentration and volume of bacteria? Make this a realistic scenario.5. You are working at the local health department as a microbiologist. A number of people attest that chicken they bought at Mytummyhurts Grocery smells rotten. You take 50 gm (this is equal to ~ 55 ml) of the chicken, place it in a volume of 450 ml dH2O, and liquefy it. What is the dilution in simplest terms?6. If you had a liquid culture of Staphylococcus aureus and transferred 2 ml to test tube "R", which contains 6 ml of nutrient broth, what would be the final dilution (in simplest terms) of the culture in test tube "R"? From the culture in "R" make a 1:8 dilution in tube "S" (this is not the final or overall dilution). Now, determine what is the final or overall dilution of the culture in tube "S"?7. Perform a 2-fold serial dilution using 4 tubes containing 1 ml of NB each and a 12 ml broth culture of Bacillus subtilis; use the same B. subtilis culture and perform a 2-fold serial dilution using 4 tubes containing 2 ml of NB each; repeat in a similar fashion using 4 tubes containing 0.5 ml each.8. I have 400 mls of an antibiotic stock solution at a concentration of 16 mg/ml. I would like a final volume of 8 mls of the antibiotic at a concentration of 4.2 mg/ml. How much of the stock solution do I need to obtain this?9. How would you prepare 21 ml of a 1:7 dilution?10. How would you prepare 16 ml of a 1:
A person eating a contaminated shellfish can lose the ability to breathe within minutes, but may be rescued if placed on a ventillator for a period of days.
Assume you have travelled back in time and entered the body of your favorite monk. You find yourself in his garden observing red and white flowering pea plants.
Why benefits are strategically significant to employers and what is some main strategic consideration. What parts of the cell did molecules in this tube probably come from? Describe briefly.
In mid afternoon, they are on it's under surface, each caterpillar hanging by a few legs. Offer a hypothesis to explain this behaviour. How would you test it.
Did the amplitude of the action potential in median giant axon vary with stimulus voltage? What is the physiological basis for your answer?
For each of the following mutagens, indicate what type of mutation will be induced. Also, explain how the mutagen induces the damage.
What is a polar molecule? Hydrogen bonding? Describe the high surface tension of water. How is this related to the proper function of the pleural serous membranes.
On the Pacific island of Guam, large herbivorous bats called "flying foxes" commonly feed on cycad seeds, a potent source of neurotoxins. The flying foxes do not visit male cones. As a result, what should be true.
What are some potential research questions that would be interesting to ask of the species prepare a hypothesis and an experiment to test your hypothesis.
Huntington disease is an autosomal dominant trait that causes neurodegeneration later in life. Matt, who is too young to show the phenotype of Huntington disease,
what ways are the mechanisms of action similar. CA2+, IP3, and cAMP have all been described as second messengers.
A ct scan of a forty year old woman with parathyroid adenoma and hyperthyroidism reveals extensive calcium deposits in the lungs and kidney paranchyma.
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