Reference no: EM132537735

1) consider bacteriophages that can undergo lytic and lysogenic cycles.do you think one of these approaches is more effective than the other in terms of success of the bacteriophage? explain and compare and contrast the lytic and lysogenic cycles?

2)The alternative sigma factor ( sigma N) plays an important role in the pathogenesis of pseudomonas aeruginosa controlling expression of virulence factors including biosynthesis of alginate ( an exopolysaccharide involved in biofilm formation ) as well as genes involved in synthesis of flagella. Among other infections, p. Aeruginosa plays an important role in lung infections of patients with cystic fibrosis. You isolate a mutant defective in the gene coding for the sigma N such that it does not produce this alternative sigma factor.

A) predict and explain the impact on this p. Aeruginosa mutant in terms of expression of virulence genes as well as the likely direct impact related to genes involved in alginate biosynthesis and flagellar structures.

B) for the purpose of study how might you restore function to this sigma N mutant i.e., what genetic approaches could you take to restore functionality to the mutant so it would behave like the wild type?

3) consider how we set up sequencing reactions for our unknowns project: we added water,the 27F ( forward) primer, and a small amount of your PCR product, which we then delivered to gene Wiz for sanger ( dideoxy) sequencing. Based on your understanding of both PCR and sanger sequencing, what do you think would have happened if we had mistakenly included both the 27F( forward ) and 1492R( reverse) primers? explain?