Consequence of performing a scratch assay

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What is the most likely consequence of performing a scratch assay with cells overexpressing GFP, wild-type Cdc42 (WT-Cdc42), or a constitutively-active Cdc42 mutant that is energy independent (CA-Cdc42) when the Cdc42 GEF is rate limiting?

a. WT-Cdc42 and CA-Cdc42 both migrate slower than GFP

b.CA-Cdc42 migrates faster than GFP, whereas WT-Cdc42 migrates at the same speed as GFP

c. WT-Cdc42 and CA-Cdc42 both migrate faster than GFP

d. CA-Cdc42 migrates slower than GFP, whereas WT-Cdc42 migrates at the same speed as GFP

e. WT-Cdc42 and CA-Cdc42 both migrate at the same speed as GFP

Reference no: EM131727336

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