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A scientist wants to analyze the transcriptome of Escherichia coli. To do so, he grows E. coli in a flask, takes a sample from the culture, and extracts the nucleic acids from the sample. She then uses an enzyme called DNase to remove the DNA, leaving only RNA. The RNA is then converted to complementary DNA (cDNA) using a reverse transcriptase. This cDNA, which is more stable than the RNA from which it was synthesized, is broken into small fragments (< 400 bp), and these fragments are then sequenced. The resulting transcriptome dataset (RNASeq) contains millions of sequence fragments corresponding to the transcripts from the original sample. However, upon reviewing his lab notes, the researcher notices he did not add the recommended amount of DNase. He suspects that his dataset may contain sequences originating from DNA, in addition to those from RNA. Describe two patterns that he could look for in the data to confirm that the sequence data reflect RNA, rather than the DNA from which the RNA was transcribed. For each, describe why such a pattern would be expected for RNA, but not DNA. Note: the E. coli genome has been sequenced in its entirety, i.e., the sequences of all genes and other genetic elements are known, as is the location of these features on the chromosome.
Mrs. Smith is 50 years of age and is nervous about going through menopause. How would you explain the process to her?
Explain the classification system used in biology to organize living creatures. Pick two organisms from each Kingdom and explain why they are placed.
What are embryonic stem cells (ES)? How do embryonic stem Cells differ from Adult stem cells?
Identify, explain and describe the relationship between the mutation and Werner's syndrome
describe how uncoupling proteins work, including their effectson cellular respiration. Explain how these proteins might play a role inthermogenesis and the control of reactive oxygen species such as superoxide.
What is life? Or what is the definition of life? does it have a impact on our life's and does scientist have a impact on life?
What is the difference between the rough and smooth endoplasmic reticulum?
Prepare a Case Study for Ophthalmology (The eye) and Otorhinolaryngology (The ear, nose, and throat) Using medical terminology, eponyms, and abbreviations (where appropriate) that are associated with the area of specialty that you select
Which organism can cause food-borne intoxication.
How many of the rabbits would you expect to be homozygous spotted? how many of the rabbits would you expect to be heterozygous spotted
what applications besides restriction mapping are there for restriction enzymes? what is the lymbic system? what are the functions of it? ow mnay pairs of somatic cells do human have?
Darren operates a nusery and he wants to apply some pollon to fertilize som flowers. To what structure should he apply the pollen?
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