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The delta G for hydrolysis of the amide group from glutamine is -14.2 kJ/mol at 25C
a) Compute the keq' for this hydrolysis
b) What would be the delta G ?' and keq' for the synthesis of Gln from Glu and ammonia?
c) In E. coli actively growing on glucose, intracellular [Glu] is 4.2 mM, and [Gln] is 0.51 mM.
What concentration of NH4+ would be needed to maintain these concentrations of Glu and
Gln at equilibrium based solely on reversal of the hydrolysis reaction? Is this concentration
of NH4+ a reasonable expectation? Why?
d) What effect would coupling this Gln synthesis to the hydrolysis of ATP delta G ? ' ( -30.5 kJ/mol
at 25C) have on the delta G ?' for Gln synthesis, and thus its favorability? (The enzyme glutamine synthetase works this way.) What would the delta G ?' be for the coupled reaction?
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