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1. Severe combined immunodeficiency disease (SCID) is fatal at an early age. SCID is caused by the lack of the enzyme adenosine deaminase, which is encoded by the gene ADA. Suppose you want to use a gene therapy method to treat the disease. You have DNA that contains the ADA gene and a virus that infects human cells. Design a protocol to get the ADA gene into the viral DNA.
2. Define restriction fragment length polymorphism (RFLP) analysis. The information generated from RFLP is useful in criminal investigations. Suppose some DNA samples are taken from a crime scene, suspect, and the victim. The samples are amplified by PCR, and then subjected to RFLP analysis. Below is a gel electrophoresis of the RFLP results. D1 is from defendant 1; D2 is from defendant 2; V is from the victim; and S1, S2, and S3 are from the crime scene. Speculate on how these results would have an impact on each defendant.
3. Define genomics and proteomics. Name two techniques that are used in each field of study. Protein is translated from mRNA, which is transcribed from the gene. However, the proteome is much larger than the genome for a given organism. Give two reasons why there are more proteins than type of genes.
Appraise the effects of HD's size, speed of strategic decision making and implementation, innovation, and quality on their ability to take competitive action.
How would you achieve the same g force with two rotors, one with a radius that is longer than the other? Show your calculation or equation set up.
Explain postpartum depression and tell how it differs from the Blues and discuss How common is "Postpartum Blues," and how long does it usually last?
How many total alleles are in the population in Model 2? What is the probability of an offspring from the Model 2 population getting a dominant allele? What is the probability of an offspring from the Model 2 population getting a recessive allele?
Assuming an OD600 of 1.00 = ~ 4 x 108 bacterial cells/ml (OR the value provided by your TA), calculate the number of cells in your original undiluted bacterial suspension.
Describe the major types of connective tissues and give at least one example of the location of each type
why are algae important in nature? how are algae fungi and lichens related and how is this relationship defined?
Steve Q. is a 52-yr-old Caucasian male with a family history of alcoholism. He is 6 feet 1 inch tall and weighs 238 pounds, with much of his excess weight around his middle. He considers himself a social drinker although he has had two arrests f..
In regard to cursorial locomotion and saltatorial locomotion, discuss the similarities and differences in limbs required for each.
Chymotrypsin and trypsin cleave peptide bonds by virtually the same mechanism. Why does chymotrypsin cleave near residues like tyrosine, but trypsin does not?
How does wind increase transpiration?
you have just been hired as an information security engineer for a large multi-international corporation. unfortunately
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