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You want to fuse a cDNA sequence to the end of two tandem"HA" antibody epitope tags within the MCS-HAHA (multiple cloning site-HAHA) region of the vector shown below. When transformed into yeast this plasmid will express the fusion protein under the transcriptional control of the pGAL1 promoter as shown. The corresponding sequence of the MCS-HAHA region is shown from the EcoRI site to the Sal1 site, and the reading frame of the two HA tags is indicated (each HA epitope is designated by the underlined amino acid sequence). Design primers with XhoI and SalI sites for PCR amplification of the entire cDNA (sequence at the bottom) to clone it together, in frame, with the HAHA epitope in the vector. Calculate for both primers their respective Tm and report the sequence of temperatures and times required for one cycle of the PCR amplification.
What are the causes of gastroesophageal reflux disease (GERD)? What is the typical prognosis for GERD and how is it treated.
evaluate and contrast the electron transport systems. Include a discussion of the inputs and outputs of each ETS and also give examples of specific electron carriers.
Think about a man who has been exercising. His arterial receptors detect a rise in his blood CO2 and send this information to the brain. He requires to increase both the rate and depth of his breathing.
Explain two processes that ensure that your full future sibling is going to be genetically different from you even though you have the same parents.
Biochemical feedback regulation or end product inhibition. What are the most general neurotransmitters in the brain.
Multicellular animals have some mechanism to control cell mass that we do not understand; for in case, salamanders of different ploidies are the same size such that a haploid, a diploid, and a pentaploid organism are about the same size.
create a specific experiment to measure the rate of cellular respiration in an organism of your choice
what is Carbonic anhydrase of red blood cells. If 10g of pure carbonic anhydrase catalyzes the hydration of 0.30g CO2 in one minute at 37 degrees C under optimal conditions, calculate the turnover number of carbonic anhydrase.
Which hormone causes an increased output of enzyme-rich pancreatic juice and stimulates gallbladder contraction to free bile. Build up a network design that accommodates these requirements and keeps costs down. These training environments must hold a..
How might the choosing of the more prevalent codon
Examine how the pancreatic endocrine hormones glucagon and insulin work antagonistically to maintain homeostasis in the body, throughout times of feast and times of famine.
sketch a diagram of the circular DNA molecule, showing the locations of the restriction sites for Ecor I and BamH I , as well as distances between them.
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