Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
A crude cell-free extract of a skeletal muscle contained 32 mg protein/ml. Ten ul (microlitres) of the extract catalyzed a reaction at rate of 0.14 umole/min under standard optimum assay conditions. Fifty ml of the extract were fractionated by ammonium sulphate precipitation. The supernatant obtained after centrifugation was redissolved in 10ml. This solution was found to contain 50 mg protein/ml. Ten ul (microlitres) of this purified fraction catalyzed the reaction at a rate of 0.65 umole/min.
(a) Calculate the percent recovery of the enzyme in the purified fraction. (Yield = units of enzyme in fraction / units of enzyme in original fraction).
(b) Calculate the degree of purification obtained by the ammonium sulphate fractionation. (Specific activity = total units of enzyme in fraction / total amount of protein in a fraction)(Fold Purification = specific activity of a fraction / specific activity of original fraction).
A geneticist determines that long hair length in mink is dominant over short hair (recessive).define how his geneticist friend should advise.
A mixture of very hot and very cold water. Organize them, so your right hand is in the front of cold water, your left hand is in front of hot water, and lukewarm water is in the middle.
There are 2-genes coding for beak color in peacocks. The R allele confers an orange beak, while r codes for a red beak. At a separate locus, the Spread gene.
Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase.
Which pair will have been likely to agree that strata such as those depicted here were deposited gradually over long periods of time by subtle mechanisms that are still at work?
For double helix formation, ?G can be measured to be -54 kJ/mol at pH 7.0 in 1 M at 25 degrees C. The heat released indicates an enthalpy change of -251 kJ/mol (-60 kcal/mol)
Injection of double stranded RNA can lead to gene silencing by degradation of RNA molecules complementary to either strand of the dsRNA.
Why is it significant for clinical laboratory personnel to follow proper methods of sterilization and specimen control.
Fructose is a ketohexose monosaccharide discovered in honey, fruits, and other sources. It is also added to some foods and drinks as a sweetener.
A child is suspected of having an eating disorder. What hormones require to be assessed prior to considering that the situation is primarily psychological?
In horse, black coat colour is influenced by the dominant allele (B) and chestnut coat colour by the recessive colour (b). Trotting gait is due to dominant gene (T), pacing gait to the recessive allele (t). What colour horse would you use to find out..
How is the lifestyle of many radially symmetrical animals different from that of bilaterally symmetrical animals? Explain how the body plan complements the lifestyle using two animals as examples.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd