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bioinformatic You have been given a gene sequence, which is in RAW file format. Perform the following tasks by visually inspecting the sequence using information you were provided during lectures for Module 1, Topics 2 and 3. CCCTGGTTATTTATTGACAGGGGTATATAGTCCAGTGGTAGGCTGGATTA TATAATTTATTGAAATTTTCGATAAATTCCGTTATAATAATATTAAATCT GAAAATATTTAGTAAAAGGATGTGAGATATGTGAAGCTAAAACGCTTGTC TTTCTTCATGTTTGTTACCTTACTGGTATTTATATCTGTTTTTCCTGTTT ATGCAAACGATTTCGAAAAACATGGTACATATTATGAAATTTTTGTCAGG TCTTTTTATGACTCTGATGGTGACGGAATAGGGGATTTGAAAGGTATAAT AGAAAAACTGGATTATCTTAATGATGGAGACCCTGAAACCATTGCTGATC TGGGGGTTAATGGTATCTGGTTAATGCCTATCTTTAAATCTCCCTCCTAT CATGGCTATGATGTAACCGATTATTATAAGATTAATCCTGACTACGGGAC TCTGGAAGACTTCCATAAGCTTGTTGAGGCTGCCCATCAAAGGGGAATCA AGGTTATAATTGATTTACCCATCAATCATACCAGTGAAAGACATCCCTGG TTTCTCAAGGCTTCCCGGGATAAGAATAGTGAATACAGGGATTATTATGT CTGGGCTGGCCCCGATACCGATACCAAAGAAACCAAGTTAGATGGAGGCC GGGTCTGGCATTATTCCCCGACCGGCATGTATTATGGGTATTTCTGGAGT GGCATGCCTGATTTAAACTATAATAACCCTGAAGTTCAGGAAAAGGTTAT TGGGATAGCAAAATACTGGTTAAAACAGGGGGTTGATGGTTTCAGGCTTG ATGGAGCCATGCATATCTTCCCACCGGCCCAGTATGATAAAAACTTTACC TGGTGGGAGAAGTTCCGTCAGGAAATTGAAGAGGTAAAACCCGTTTACCT GGTGGGTGAGGTCTGGGATATTTCGGAAACGGTAGCTCCTTACTTCAAAT ATGGTTTTGATTCTACCTTTAACTTTAAACTGGCAGAGGCAGTTATCGCT ACGGCTAAAGCTGGATTTCCCTTTGGTTTTAATAAAAAGGCAAAACATAT TTACGGGGTATATGACAGGGAGGTTGGATTTGGGAATTATATCGATGCTC CCTTCCTGACCAACCATGATCAGAACCGGATTTTGGACCAGCTTGGGCAG GATCGTAATAAGGCCAGGGTTGCTGCCAGTATTTATTTGACCTTGCCTGG TAATCCCTTTATTTACTATGGTGAAGAAATCGGTATGAGGGGGCAGGGGC CCCATGAAGTTATCAGGGAGCCCTTCCAGTGGTATAATGGATCCGGGGAG GGAGAAACATACTGGGAGCCAGCCATGTATAATGATGGCTTTACTTCTGT TGAACAGGAAGAAAAGAATCTCGATTCCCTCTTAAATCACTACAGGAGGT TAATCCATTTCCGGAACGAAAATCCTGTCTTTTATACCGGTAAGATTGAG ATTATAAATGGAGGATTAAATGTAGTTGCATTTAGAAGATATAATGATAA GAGGGATTTATATGTCTACCATAACCTGGTAAACAGACCGGTTAAAATAA AAGTGGCTAGTGGTAACTGGACCTTATTGTTTAATTCAGGTGATAAGGAA ATTACCCCTGTTGAAGATAATAATAAACTTATGTATACTATCCCTGCTTA TACTACCATTGTTCTGGAAAAGGAGTAAAGGGAGAGGGTGAGTAATAATT ATGAAAAGGAGAGGGTAAATCCCTCTCCTTTTTTGTTAAA (a) Identify the possible Open Reading Frame (ORF). Hint: search for the START and STOP codons. (b) How many amino acids will the gene code for? (c) Translate the sequence and deduce the amino acid sequence for the putative protein. (d) Can you identify a signal peptide? If yes, identify it? (e) Sometimes, it is not possible to identify a gene using the method you used in question (a) above. One of the reasons is that the gene has not been sequenced completely - this happens during random sequencing of clones from bacterial genomic libraries. Perform a 6 frame translation of the given gene. Can you see the difference between the amino acid composition of all 6 translations. (f) Determine the G+C content of the coding region of the gene. Change the G+C content of the gene without changing the amino acid composition.
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How did you weigh the different pieces of evidence to reach a conclusion to questions (2) and What evidence was most important, what was least important, etc.?
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The human appendix, a vestigial extension off the large intestine, is homologous to a structure called a caecum in other mammals.
Consider human subships with 4 children and assume that each birth is equally likely to result in a boy (B) or a girl (G)?
What is a population? What is a species? You are conducting fieldwork and discover two populations of squirrels living on opposite sides of a lake. How would you design a study to determine, without disturbing the squirrels, whether these two populat..
1. where does translation occur?a. in the nucleusb. cytoplasmc. on the dnad. none of above2. select the correct answer
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