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A generalized transducing phage is used to transduce an a-b- c- d- e- recipient strain of E. coli with an a+ b+ c+ d+ e+ donor. The recipient culture is plated on various media with the results shown in the following table. (Note that a- indicates a requirement for A as a nutrient, and so forth.) What can you conclude about the linkage and order of the genes?
Compounds added Presence (+) or to minimal medium absence (-) of colonies
CDE -
BDE -
BCE +
BCD +
ADE -
ACE -
ACD -
ABE -
ABD +
ABC -
Does this indicate that our original estimate of 350 individuals was too low or too high? Explain your answer in terms of the principles that underlie mark recapture method.
Explain the reverse transcription reaction (what are the components of the reaction? What are the possible primers for the reaction and how do they differ from each other?)
Determine which of the following statements is accurate regarding the evolution of drug resistance in a virus?
Assume that the rate of sea-floor spreading was constant during the 1-million-year period depicted above, what have to be the approximate age of marine fossils found in undisturbed sedimentary rock instantly overlying the igneous rock at the arrow la..
Assume you work in a cool professor's Drosophila lab and you are told to figure a genetics problem regarding fly hairness.
The following 3-recessive genes are found in corn. Brittle endosperm, glossy leaf, and ragged seedling. A tri-hybrid is test-crossed producing the following offspring.
Explain the four categories of amino acid side chains. Determine what types of interactions are the members of each of these categories likely to make?
The -10 and -35 sequences in bacterial promoters are separated by about two turns of the DNA double helix (remember genetics?). How do you think transcription would be affected if the deletion were introduced such that the -35 sequence was moved to -..
Compute the change in energy of an atom that emits a photon of wavelength 2.21 meters. (Planck's constant is 6.626 x 10-34 joule seconds; the speed of light is 2.998 x 108 m/s)
Would the passive movements of substances occur if an interstitial solute concentration was the same as the filtrate solute concentration.
How many pairs of genes regulating tail length character. Adding of H+ or OH- to a reaction increased its overall rate indicating the rate limiting step was the addition of H+ or OH- to the intermediate. Is this really true? Why or why not.
What is most likely genotype of the father? Mother? If the seventh child had blue eyes what could you conclude about parents genotyoes. What possible dissimilarity between these forms would give chemists a clue that open-chain form was not present.
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