Assay the amount of a chemical in the plasma

Reference no: EM13533649

To assay the amount of a chemical in the plasma of a patient anenzyme specific for the chemical is added to a sample of the plasmaand the rate of product formation is measured. The enzyme hasa Km of 30 uM (micromolar). The process is: 0.1 ml of theenzyme solution is added to 1.9 ml of plasma and the rate ofproduct formation determined. The rate was 10-2 uM/sec. Thisresult is produced by a concentration of 15 uM of the chemical. Asecond assay was run with 0.l ml of enzyme solution placed in 3.9ml of plasma. What rate of product formation would you predict?

Reference no: EM13533649

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