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SNAREs exist as complementary partners that carry out membrane fusions between appropriate vesicles and their target membranes. In this way, a vesicle with a particular variety of v-SNARE will fuse only with a membrane that carries the complementary t-SNARE. In some instances, however, fusions of identical membranes (homotypic fusions) are known to occur. For example, when a yeast cell forms a bud, vesicles derived from the mother cell’s vacuole move into the bud where they fuse with one another to form a new vacuole. These vesicles carry both v-SNAREs and t-SNAREs. Are both types of SNARE essential for this homotypic fusion event?
When looking for a gene of interest, we often scan cDNA libraries. What is the difference between a cDNA and a genomic library? Can you think of situations in which we would probe a genomic library?
Which precautions would you follow to preserve Bacillus subtilis and what are the physical and chemical hazards of handling Bacillus subtilis?
What procedure ensures that pre-mRNA processing is co-transcriptional? Disucss ow are all three steps of processing sequentially controlled?
Other possible sources of the infection included the woman's prior sexual contacts and occupational exposure, given that she was a nurse.
They have one child. What is the possibility that their child will have the disease. 52 dark moths and 21 light moths were at random recaptured. Which would be the most reasonable assumption.
Explain the roles of the CAP binding site and the operating site in relationship to the 3 proteins required for growth on lactose. Why aren't these proteins produced all the time? Explain the differences in function when glucose is absent and lact..
Many enzymes used by molecular biologists were isolated from the bacterium Escherichia coli, which is an inhabitant of the human gut. Most of these enzymes are used at a temperature of 37 degrees Celsius.
Develop the process of transcription using dsDNA as a template to produce a final functional mRNA. In your drawing label the direction of the nucleic acids, RNA polymerase,
What is the name of the process in bacteria that involves DNA transfer through a cytoplasmic connection known as a pilus?
1. Begin with the information in DNA, a specific gene. Describe how this genetic information results in the formation of a protein.
Arrange the amino acid substitutions in increasing order of conservatism and amino acids are given using one letter codes to give you some practice in using those.
How do you calculate the energy yield of sugars, amino acids and fatty acids? If you could explain or if it is too much refer me to one I am horribly confused. Thank you!
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