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Acetyl-CoA carboxylase is a key regulatory enzyme in fatty acid synthesis. Early studies on the molecular properties of this enzyme purified from avian liver produced the data given in table.
Table: Molecular properties of avian acetyl-CoA carboxylase
Treatments
Sedimentation coefficient (S)
Molecular mass (kDa)
Catalytic activity (arbitrary units)
10 mM citrate
55-59
4000-8000
35
0.5M NaCl, pH8-9
13.1
410
2.5
a) Explain the results shown in table in terms of the molecular structure of acetyl-CoA carboxylase and comment on their apparent physiological significance.
b) Which cellular compartment would you expect the acetyl-CoA carboxylase activity to have been observed in and why?
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