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Q. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is additional to a mixture containing EcoRI and bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and after that added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
Bacteria contain a plasmid into which eukaryotic gene has integrated will grow in what?
The patient lives near the San Luis Rey River and has no history of foreign travel, blood transfusion, or intravenous drug use. What is the disease? How was it obtained.
In an experiment you are studying a neuron that receives synaptic inputs from two other neurons: one from neuron A and one from neuron B. Illustrate a separate graph for each value of Vm.
Describe how the cell derives additional energy by switching . What was the key factor that determined this change.
Assuming that genotype essential to produce flat tails is homozygous recessive (such as bb) compute the frequency of the b allele in the squirrel population to two decimal places.
What hormone do the adrenal glands produce. An uncold-worked brass specimen of average grain size 0.008 mm has yield strength of 170 MPa. Estimate the yield strength of this alloy (in MPa) after it has been heated to 600°C for 1260 s, if it is known ..
The results of these experiments point to that plants can be genetically transformed for resistance to virus disease development. What is an expected outcome of a cross between a homozygous recessive woman and a man with hypophosphatemia.
What advantages do the O-Scale System and the Canadian Physical Activity, Fitness and Lifestyle Approach-Health Body Composition Assessment procedure has in evaluation to percent body fat predictions from skin folded measurements, when used in indivi..
Clarify the effects of the ecb infestation you used. Were all corn varieties evenly effective at controlling the ecb? How do you know.
In bacterium that possesses antibiotic resistance and potential to persist through very adverse conditions, such as freezing, drying, or high temperatures, DNA have to be located within, or be part of, which structures.
A nearsighted woman (Nn) with hazel eyes (Hh) marries the man with normal vision and hazel eyes (Hh). Their three children all have blue eyes and normal vision. What is the possibility that their next child will have blue eyes and be nearsight.
Your assignment, if you want to accept this, is to cross two of the F1 offspring and complete Punnett square for this cross and then write the resulting genotype and phenotypes. This must be 100 percent completely done for any extra credit. Your cros..
Sketch a diagram that illustrates how you would expect the triglyceride and phospholipid molecules to interact with the water. Be sure to indicate structures, if any that might form as a result of these interactions.
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