Concentration of the Analyte Assignment Help

Assignment Help: >> Deviations from Beer-Lambert’s Law - Concentration of the Analyte

Concentration of the Analyte:

As per Beer and Lambert's law, the plot of absorbance versus the concentration of absorbing substance should be a directly line while ε and b are constant. The path length could always be held constant but there are a few factors that affect ε and it is found which at high concentration, ε is not constant. Thus, at higher concentrations (>10-3 mol dm3) there might be deviation from the law.

Temperature

The temperature is not considered as a significant factor since ordinarily the measurements are made at a constant temperature. Therefore, changes in temperature sometimes might shift ionic equilibrium and the absorptivity. For instance, the colour of acidic ferric chloride solution changes from yellow to reddish brown on heating because of change in λmax and absorptivity.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd