Shoot multiplication -clonal propagation, Biology

Shoot Multiplication -Clonal Propagation

This is the most important step with respect to the rate of propagation and genetic uniformity of the product. The most reliable and, therefore, the most popular method of shoot multiplication is forced proliferation of axillary shoots. For this, cultures are initiated from apical or nodal cuttings carrying one or more vegetative buds. In the presence of a cytokinin alone or in combination with a low concentration of an auxin, such as IAA or NAA, the pre-existing buds grow and produce 4-6 shoots (sometimes up to 30-40 shoots) within 3-4 weeks. By periodic removal of individual shoots and planting them on fresh medium of the original composition, the shoot multiplication cycle can be repeated almost indefinitely, and a stock of large number of shoots built up in a short period of time.

Treatments with PGRs as described above can also help in a rapid buildup of shoots by inducing adventitious buds by the explant directly or after callusing. Somatic embryogenesis, which generally occurs after callusing of the explant, is another method of micro propagation. Somatic embryogenesis is not only fast, but may also allow partial automation of micro propagation and the propagules so produced (somatic embryos) bear both, shoot and root meristems. However, adventitive differentiation of shoots or somatic embryos, especially from callus tissue, has the risk of genetic variability in the progeny. Such variation, that develops in tissue culture called "somaclonal variation" is not desirable for micro propagation but is being exploited as a novel source of useful variations for crop improvement.

Posted Date: 1/24/2013 5:28:31 AM | Location : United States







Related Discussions:- Shoot multiplication -clonal propagation, Assignment Help, Ask Question on Shoot multiplication -clonal propagation, Get Answer, Expert's Help, Shoot multiplication -clonal propagation Discussions

Write discussion on Shoot multiplication -clonal propagation
Your posts are moderated
Related Questions
Q. Show the Paleobotanical Evidence? Paleobotany deals with the study of fossil records of plants and animals. New techniques and approaches to the study of fossil flowering pl

Structure of the Vascular Cambium This meristem, in the anatomical sense of the term usually includes two histologically distinct kinds of cells: i) Fusiform Initials, i

Q. What is the destination of the notochord in protochordates and in vertebrates? In vertebrates the notochord disappears and gives birth to the spinal column known as vertebra

Graphs are a way to convey information and trends in a convenient fashion. For this week's Discussion Board you should search the internet for a graph related to biology. Provide u

True or false, adaptations are the result of finding the best or only solution to a given problem a species faces.

Tolerance Range - Ecosystem Organisms are able to survive only within certain maximum and minimum limits with respect to each environmental factor such as water, light and tem

L YMPH A TI C SYSTEM As 'Middle man' of circulation it mediates exchange between blood and the tissue. It is also a collecting system like venous system. Basically 'o

osmoregulation in non chordates

Tuberculosis Tuberculosis is a chronic infectious disease which is caused by a bacterium - Mycobacterium tuberculosis.  It affects the lungs most commonly but call gel localize

Define Post-surgical Nutrition Support for CHD Patient? Nutrition support should be started as soon as possible. If oral not possible, enteral nutrition support should be pr