Shoot multiplication -clonal propagation, Biology

Shoot Multiplication -Clonal Propagation

This is the most important step with respect to the rate of propagation and genetic uniformity of the product. The most reliable and, therefore, the most popular method of shoot multiplication is forced proliferation of axillary shoots. For this, cultures are initiated from apical or nodal cuttings carrying one or more vegetative buds. In the presence of a cytokinin alone or in combination with a low concentration of an auxin, such as IAA or NAA, the pre-existing buds grow and produce 4-6 shoots (sometimes up to 30-40 shoots) within 3-4 weeks. By periodic removal of individual shoots and planting them on fresh medium of the original composition, the shoot multiplication cycle can be repeated almost indefinitely, and a stock of large number of shoots built up in a short period of time.

Treatments with PGRs as described above can also help in a rapid buildup of shoots by inducing adventitious buds by the explant directly or after callusing. Somatic embryogenesis, which generally occurs after callusing of the explant, is another method of micro propagation. Somatic embryogenesis is not only fast, but may also allow partial automation of micro propagation and the propagules so produced (somatic embryos) bear both, shoot and root meristems. However, adventitive differentiation of shoots or somatic embryos, especially from callus tissue, has the risk of genetic variability in the progeny. Such variation, that develops in tissue culture called "somaclonal variation" is not desirable for micro propagation but is being exploited as a novel source of useful variations for crop improvement.

Posted Date: 1/24/2013 5:28:31 AM | Location : United States







Related Discussions:- Shoot multiplication -clonal propagation, Assignment Help, Ask Question on Shoot multiplication -clonal propagation, Get Answer, Expert's Help, Shoot multiplication -clonal propagation Discussions

Write discussion on Shoot multiplication -clonal propagation
Your posts are moderated
Related Questions
Planning of Nursing Care Planning of the nursing care depends on the decision of the surgery. The surgery may be performed in two stages depending upon the degree of the d

Analysis of Developmental Potency of Nuclei The instances given above and many other observations and experimental results of this nature provide tough support to the hypothes

A) Maintain structural integrity of the cell B) Regulate the movement of substances into and out of the cell C) Provide communication between the cells D) ALl of the above

Examples of cloning and molecular biology cloning may produce heterozygous individuals (as mother was heterozygous) and suggests a way to get pure breeding cats molecu

In shorthorn cattle, the coat colours red or white are controlled by a single pair of alleles. A calf which receives the allele for red coat from its mother and the allele for whit

Q. What are the stages of mitosis? Mitosis involves replication of DNA and its separation into two new daughter cells. Whereas only four phases of mitosis are often listed. Ent


Draw a diagram of transverse section of mammalian bone and label the following parts in it: (i) Haversian canal (ii) Lacuna containing osteocytes (iii) Canaliculi

What is the difference between the concepts of food chain and food web? The chain theory is a theoretical model to study the energy flux in ecosystems. In fact, in an ecosystem

These are safe and free of systemic side effects. However, gastrointestinal side effects are common, and compliance is poor. The average LDL decreases by approximately 15 per cent