Production of disease-free plants, Biology

Production of Disease-free Plants

Under normal conditions plants are infected by a wide range of pathogens such as bacteria, fungi, viruses, viroids, and insects like nematodes and insects. Many perennial plants and those propagated by vegetative means are systematically infected with one or more pathogens, which reduce yield, vigour and quality of the plant. If explants for micropropagation are derived from an infected plant, the pathogens can multiply and spread to a large number of plants.

It is, therefore, essential to use disease free stock plants for micropropagation. Eradication of viruses and other pathogens is also desirable from the point of view of international exchange of plant materials. Whereas bacteria and fungi present on the surface of the plant material can be easily eliminated by treatment with surface sterilizing agents, there is no dependable treatment against viruses. Viruses can multiply within the shoots in-vitro without symptoms. Traditionally, thermotherapy has been used for virus elimination but it is not effective against all viruses. Moreover, heat treatment may adversely affect the plant tissues. For some reasons viruses are unable to enter or survive in the apical meristems. Therefore, even in infected plants the apical meristems are generally free of viruses. Taking advantage of this observation, Morel (1950) developed the technique of shoot tip culture to raise virus-free plants from infected individuals. Since then it has become the most effective method of virus elimination. It involves excision of 0.5-1 mm long shoot tips, including apical meristem and one or two leaf primordia and their cultivation on a suitable medium to regenerate whole plants.

Posted Date: 1/24/2013 5:24:44 AM | Location : United States







Related Discussions:- Production of disease-free plants, Assignment Help, Ask Question on Production of disease-free plants, Get Answer, Expert's Help, Production of disease-free plants Discussions

Write discussion on Production of disease-free plants
Your posts are moderated
Related Questions

who is the first person who saw the cell

The movement of the chromosome is called anaphase A, and the extension of the poles is termed anaphase B.The mechanism of these movements are discussed below. Chromosome move

SURGERY FOR CORONARY ARTERY DISEASE : Stenotic coronary artery disease (CAD) is caused by the thickening and narrowing of the coronary arteries (Atherosclerosis). Initially it cau

There are three natriuretic peptides-atrial (ANP) stored mainly in the atrium, brain (BNP) stored mainly in the ventricular myocardium and C-natriuretic peptide (CNP) located prima

What is Rationale for Menu Planning? 'Menu', as we all know, is nothing but a list of dishes planned for preparation, and forms the very core of all activities in the food serv

Early campaigns of the 19th century that focused on sanitation, hygiene, housing, and nutrition had little effect in controlling communicable disease due to flawed rationale based

What is an example of an ion of biological importance? Sodium in one, but so are potassium and calcium

Determine some assessment measures of Zinc? Measurement of zinc in plasma: This is the most common method. Fasting concentrations of less than 70 μg/litre suggests deficie

Dfine Integrated Child Development Services (ICDS)? The adolescent girl scheme under ICDS intends to cover school dropout girls, 11-18 years in age with a view to meet their ne