Microinjection method for gene transfer, Biology

Microinjection method: In another approach fertilised eggs are microinjected by DNA. Microinjection requires excess of fertilized eggs. Gene transfer by microinjection is the predominant method used to produce transgenic farm animals. Since the insertion of DNA results in a random process, transgenic animals are mated to ensure that their offspring acquire the desired transgene. However, the success rate of producing transgenic animals individually by these methods is very low and it may be more efficient to use cloning techniques to increase their numbers. For example, gene transfer studies revealed that only 0.6% of transgenic pigs were born with a desired gene after 7,000 eggs were injected with a specific transgene.

To obtain good number of eggs, donor female is induced to superovulate and fertilised eggs are obtained after mated with male. In this method, the novel gene in an appropriate copy number is directly introduced into the male pro-nucleus of a fertilised egg just before its fusion with female pro-nucleus. Male pronucleus is clearly visible as it is larger than the female pronucleus in the fertilised egg.   The fertilised egg carrying transgenes are implanted into receptive female (foster mother). Implanted females or foster mother gives birth to progenies carrying transgene. Finally the animals which carry transgenes are identified and bred to establish transgenic lines.  This method is still the widely accepted method in mouse and rabbit model, however, in farm animals, opaqueness of zona pellucida and lack of clear vision of male pronucleus are the main limitations. Further random integration of gene in host genome may require longer time to produce a pure line of transgenic livestock.

Posted Date: 9/18/2012 7:50:20 AM | Location : United States







Related Discussions:- Microinjection method for gene transfer, Assignment Help, Ask Question on Microinjection method for gene transfer, Get Answer, Expert's Help, Microinjection method for gene transfer Discussions

Write discussion on Microinjection method for gene transfer
Your posts are moderated
Related Questions
What are some factors that can lead to protein denaturation? Protein denaturation can be caused by temperature variation, pH change, alters in the concentration of surrounding

Define Dietary modifications in the diet of the elderly? Loss of teeth due to increased decay of teeth and gums is common in aged person. Fitting dentures may also make chewin

What are the main water properties that make water special for life? The water properties that make water biologically significant are molecular polarity, thermal stability (el

Define Factors Influencing RS Content of Foods? Different fractions of RS mentioned in Table may be naturally present in some foods or may be generated as a result of industria

T h e i l e r io s i s It is also referred as tropical theileriosis and results in fever, enlargement of superficial lymphnodes, anaemia and jaundice. E tiolo

Define Absorption, Storage and Elimination of Pyridoxine? Pyridoxine, pyridoxal and pyridoxamine (along with their phosphorylated forms) occur in plant and animal foods. The ph

Explain the cell cycle in details? Most cells of higher organisms follow a cyclic pattern of division. The bulk of the cycle consists of the stage known as interphase, the peri

Methods Useful in Self-Incompatibility More specific methods useful in overcoming self-incompatibility are mentioned below 1. Bud Pollination : In Petunia axillaris early


Mitochondria Mitochondria are commonly known as the "power house" of cells. These are the distinct organelles of all eucaryotic cells, plants, fungi and some protists. In the