Microinjection method for gene transfer, Biology

Microinjection method: In another approach fertilised eggs are microinjected by DNA. Microinjection requires excess of fertilized eggs. Gene transfer by microinjection is the predominant method used to produce transgenic farm animals. Since the insertion of DNA results in a random process, transgenic animals are mated to ensure that their offspring acquire the desired transgene. However, the success rate of producing transgenic animals individually by these methods is very low and it may be more efficient to use cloning techniques to increase their numbers. For example, gene transfer studies revealed that only 0.6% of transgenic pigs were born with a desired gene after 7,000 eggs were injected with a specific transgene.

To obtain good number of eggs, donor female is induced to superovulate and fertilised eggs are obtained after mated with male. In this method, the novel gene in an appropriate copy number is directly introduced into the male pro-nucleus of a fertilised egg just before its fusion with female pro-nucleus. Male pronucleus is clearly visible as it is larger than the female pronucleus in the fertilised egg.   The fertilised egg carrying transgenes are implanted into receptive female (foster mother). Implanted females or foster mother gives birth to progenies carrying transgene. Finally the animals which carry transgenes are identified and bred to establish transgenic lines.  This method is still the widely accepted method in mouse and rabbit model, however, in farm animals, opaqueness of zona pellucida and lack of clear vision of male pronucleus are the main limitations. Further random integration of gene in host genome may require longer time to produce a pure line of transgenic livestock.

Posted Date: 9/18/2012 7:50:20 AM | Location : United States







Related Discussions:- Microinjection method for gene transfer, Assignment Help, Ask Question on Microinjection method for gene transfer, Get Answer, Expert's Help, Microinjection method for gene transfer Discussions

Write discussion on Microinjection method for gene transfer
Your posts are moderated
Related Questions
Q. What is Angina Pectoris? Chest discomfort is often reported by most patients especially those which are chronic cases of dyslipidemia and/or hypertension. Like diarrhoea and

What is extracellular digestion? Extracellular digestion is that in which food breaking into utile molecules that can be internalized by the cell is completed in the extracellu

identifying characters of pennetula

What is Concentration Gradient? The graduated dissimilarity in concentration of a solute per unit - distance by a solution.

Why lymph is also called middle man of body

Concerning the mixture of arterial with venous blood what is the difference between the human fetal circulation and the adult circulation? In the human fetal circulation there

Contagious ecthyma The contagious ecthyma virus is immunologically distinct from vaccinia, but similar  to the causative agent of pseudo-cow-pox. It belongs to the genus Parapoxvi

DIFFERENCE S BETWEEN MYOSIN AND ACTIN FILAMENTS     Myosin filaments (primary filaments)   Actinfilament s (secondary filaments)

What are the basic constituents of the cell membrane? The cell membrane is produced of lipids, proteins and carbohydrates. The membrane lipids are phospholipids, a special t

What is nucleus where it is found? Nucleus :  The nucleus is present only in eukaryotic cells. It is the control center and is generally found in the center of the cell. The