Microinjection method for gene transfer, Biology

Microinjection method: In another approach fertilised eggs are microinjected by DNA. Microinjection requires excess of fertilized eggs. Gene transfer by microinjection is the predominant method used to produce transgenic farm animals. Since the insertion of DNA results in a random process, transgenic animals are mated to ensure that their offspring acquire the desired transgene. However, the success rate of producing transgenic animals individually by these methods is very low and it may be more efficient to use cloning techniques to increase their numbers. For example, gene transfer studies revealed that only 0.6% of transgenic pigs were born with a desired gene after 7,000 eggs were injected with a specific transgene.

To obtain good number of eggs, donor female is induced to superovulate and fertilised eggs are obtained after mated with male. In this method, the novel gene in an appropriate copy number is directly introduced into the male pro-nucleus of a fertilised egg just before its fusion with female pro-nucleus. Male pronucleus is clearly visible as it is larger than the female pronucleus in the fertilised egg.   The fertilised egg carrying transgenes are implanted into receptive female (foster mother). Implanted females or foster mother gives birth to progenies carrying transgene. Finally the animals which carry transgenes are identified and bred to establish transgenic lines.  This method is still the widely accepted method in mouse and rabbit model, however, in farm animals, opaqueness of zona pellucida and lack of clear vision of male pronucleus are the main limitations. Further random integration of gene in host genome may require longer time to produce a pure line of transgenic livestock.

Posted Date: 9/18/2012 7:50:20 AM | Location : United States







Related Discussions:- Microinjection method for gene transfer, Assignment Help, Ask Question on Microinjection method for gene transfer, Get Answer, Expert's Help, Microinjection method for gene transfer Discussions

Write discussion on Microinjection method for gene transfer
Your posts are moderated
Related Questions
Abscisic Acid Abscisic acid (ABA) as a naturally occurring growth inhibitor was discovered through independent investigations of different physiological phenomena in two diffe

F ALLOPIAN TUBE - Fertilisation occur in it. 10-12 cm long muscular tube. It is supported by double fold of peritonium. It shows 4 regions - (i ) Infundibulum - Bro

Sucrose is commonly used to preserve fruits why not glucose? Ans) Glucose is reactive due to its open-chain form having an aldehyde group.

Define Thermal Coagulation and Film Formation? 1. Thermal Coagulation and Film Formation: Concentrated soy proteins can be thermally coagulated on a flat metallic surface, such

The description and measurement of circadian rhythms. Describe the standard method used for the recording and graphing of behavioral rhythms in animals, especially the use of

Suppuration High number of PMN cells have been detected around implants that are associated with severe signs of mucosal inflammation. Several studies have shown the presence o

Explain the Food Applications of guar Guar imparts smoothness to ice cream by promoting small ice crystals during the freezing process. Guar gum in the dressing of cot

Posterior Maxilla Posterior maxilla has very poor quality of bone demanding special consideration in implant placement:  Increasing the number of implants to decrease the cr

Q. What are the few examples of the energetic function of organic molecules? Ans. Since they are complex molecules, organic molecules store large amount of energy, present

Define Protein Requirements for physical fitness? Proteins provide energy to the body. Since exercise may increase an athlete's need for protein, depending on the type and freq